PA5-27546

antibody from Invitrogen Antibodies

Targeting: RPS15 MGC111130, RIG, S15

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-27546

Provider

Invitrogen Antibodies

Product name

RPS15 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant protein fragment

Description

Recommended positive controls: U87-MG, SK-N-SH, IMR32, SK-N-AS, MCF-7, MDA-MB-231.

Concentration

0.18 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of RPS15 using A) 30 µg U87-MG whole cell lysate (B) 30 µg SK-N-SH whole cell lysate (C) 30 µg IMR32 whole cell lysate and D) 30 µg SK-N-AS whole cell lysate. Samples were loaded onto a 12% SDS-PAGE gel and probed with a RPS15 polyclonal antibody (Product # PA5-27546) at a dilution of 1:1000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of RPS15 using A) 30 µg MCF-7 and B) 30 µg MDA-MB-231. Samples were loaded onto a 12% SDS-PAGE gel and probed with a RPS15 polyclonal antibody (Product # PA5-27546) at a dilution of 1:1000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

RPS15 Polyclonal Antibody detects RPS15 protein by Western blot analysis. A. 30 µg MCF-7 whole cell lysate/extract. B. 30 µg MDA-MB-231 whole cell lysate/extract. C. 30 µg IMR32 whole cell lysate/extract. D. 30 µg SK-N-AS whole cell lysate/extract.12 % SDS-PAGE. RPS15 Polyclonal Antibody (Product # PA5-27546) dilution: 1:1,000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

RPS15 Polyclonal Antibody detects RPS15 protein by Western blot analysis. A. 30 µg U87-MG whole cell lysate/extract. B. 30 µg SK-N-SH whole cell lysate/extract. C. 30 µg IMR32 whole cell lysate/extract. D. 30 µg SK-N-AS whole cell lysate/extract.12 % SDS-PAGE. RPS15 Polyclonal Antibody (Product # PA5-27546) dilution: 1:1,000.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of RPS15 in paraformaldehyde-fixed MCF-7 cells using a RPS15 polyclonal antibody (Product # PA5-27546) at a 1:500 dilution.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

RPS15 Polyclonal Antibody detects RPS15 protein at cytosol on mouse hind brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse hind brain. RPS15 Polyclonal Antibody (Product # PA5-27546) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

RPS15 Polyclonal Antibody detects RPS15 protein at cytosol on mouse liver by immunohistochemical analysis. Sample: Paraffin-embedded mouse liver. RPS15 Polyclonal Antibody (Product # PA5-27546) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

RPS15 Polyclonal Antibody detects RPS15 protein at cytosol on mouse prostate by immunohistochemical analysis. Sample: Paraffin-embedded mouse prostate. RPS15 Polyclonal Antibody (Product # PA5-27546) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.