NBP2-61237

antibody from Novus Biologicals

Targeting: CHMP7 MGC29816

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Antibody Data

Product number

NBP2-61237

Provider

Novus Biologicals

Product name

Rabbit Polyclonal CHMP7 Antibody

Antibody type

Polyclonal

Description

Antigen Affinity-purified.

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

0.1 ml

Storage

Store the unopened product at 2 - 8degrees C. Protect from light. Do not use past expiration date.

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: CHMP7 Antibody [NBP2-61237] - Detection of human CHMP7 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-CHMP7 antibody NBP2-61237 used for IP at 20 ul per reaction. CHMP7 was also immunoprecipitated by rabbit anti-CHMP7 antibody NBP2-61238. For blotting immunoprecipitated CHMP7, NBP2-61237 was used at 1:250. Detection: Chemiluminescence with an exposure time of 30 seconds.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: CHMP7 Antibody [NBP2-61237] - Detection of human CHMP7 by western blot. Samples: Whole cell lysate (15 ug) from HeLa, 293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-CHMP7 antibody NBP2-61237 used for WB at 1:1000. Detection: Chemiluminescence with an exposure time of 3 minutes.