Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [4]
- Immunohistochemistry [1]
- Other assay [1]
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Validation data
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- Product number
- PA5-36059 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- HSP105 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- This antibody detects endogenous protein at a molecular weight of 105 kDa.
- Concentration
- 1 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of HSP105 in Lane 1: HEK293T cell lysate, Lane 2: NIH-3T3 cell lysate, Lane 3: PC12 cell lysate. Samples were incubated with HSP105 polyclonal antibody (Product # PA5-36059) at a dilution of 1:500.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of HSPH1/HSP105 using HSPH1/HSP105 polyclonal antibody (Product # PA5-36059) at a dilution of 1:500. Lane 1: HEK293T cell lysate, Lane 2: NIH-3T3 cell lysate, Lane 3: PC12 cell lysate.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of HSP105 in Lane 1: HEK293T cell lysate, Lane 2: NIH-3T3 cell lysate, Lane 3: PC12 cell lysate. Samples were incubated with HSP105 polyclonal antibody (Product # PA5-36059) at a dilution of 1:500.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of HSP105 in Lane 1: MCF-7 whole cell lysate (40 µg), Lane 2: PC12 whole cell lysate (40 µg), Lane 3: the lung tissue lysate of mouse (30 µg), Lane 4: the lung tissue lysate of rat (30 µg). Samples were incubated with HSP105 polyclonal antibody (Product # PA5-36059) at a dilution of 1:1,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of HSPH1/HSP105 in paraffin-embedded human breast carcinoma using HSPH1/HSP105 polyclonal antibody (Product # PA5-36059) at a dilution of 1:100.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoprecipitation of HSP105 in L02 cell lysate (sepharose bead conjugate, lane 2 and lane 3) and Nonspecific IgG Control (sepharose bead conjugate, lane 4). Lane 1 is 20% input. Samples were incubated with HSP105 polyclonal antibody (Product # PA5-36059) Results legends show supernatant (upward arrow) and deposition (downward arrow).