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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
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- Validations
- Western blot [1]
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- Product number
- MAB2035 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#MAB2035, RRID:AB_1674776
- Product name
- HSPH1 monoclonal antibody, clone J1G12
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody raised against partial recombinant HSPH1.
- Isotype
- IgG
- Antibody clone number
- J1G12
- Storage
- Store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references Different localization of Hsp105 family proteins in mammalian cells.
Hsp105 family proteins suppress staurosporine-induced apoptosis by inhibiting the translocation of Bax to mitochondria in HeLa cells.
Characterization of heat shock protein 110 and glucose-regulated protein 170 as cancer vaccines and the effect of fever-range hyperthermia on vaccine activity.
Saito Y, Yamagishi N, Hatayama T
Experimental cell research 2007 Oct 15;313(17):3707-17
Experimental cell research 2007 Oct 15;313(17):3707-17
Hsp105 family proteins suppress staurosporine-induced apoptosis by inhibiting the translocation of Bax to mitochondria in HeLa cells.
Yamagishi N, Ishihara K, Saito Y, Hatayama T
Experimental cell research 2006 Oct 15;312(17):3215-23
Experimental cell research 2006 Oct 15;312(17):3215-23
Characterization of heat shock protein 110 and glucose-regulated protein 170 as cancer vaccines and the effect of fever-range hyperthermia on vaccine activity.
Wang XY, Kazim L, Repasky EA, Subjeck JR
Journal of immunology (Baltimore, Md. : 1950) 2001 Jan 1;166(1):490-7
Journal of immunology (Baltimore, Md. : 1950) 2001 Jan 1;166(1):490-7
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- Experimental details
- Cell lysate of HeLa (30 ug) was resolved by SDS-PAGE and probed with HSPH1 monoclonal antibody, clone J1G12 (Cat # MAB2035) (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
