M04168-1

antibody from Boster Biological Technology

Targeting: HSPH1 HSP105A, HSP105B, KIAA0201, NY-CO-25

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Data presented on provider website)

Immunohistochemistry (Data presented on provider website)

Flow cytometry (Data presented on provider website)

Antibody Data

Product number

M04168-1

Provider

Boster Biological Technology

Product name

Anti-Hsp105/HSPH1 Antibody Picoband™ (monoclonal, 3D10)

Antibody type

Monoclonal

Description

Mouse IgG monoclonal antibody for Hsp105/HSPH1 detection. Tested with WB, IHC-P, ICC/IF, FCM in Human.

Reactivity

Human

Host

Mouse

Isotype

IgG

Antibody clone number

3D10

Vial size

100μg/vial

Storage

At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquoted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.

Handling

Add 0.2ml of distilled water will yield a concentration of 500μg/ml.

Western blot

Supportive validation

Submitted by

Boster Biological Technology (provider)

Main image

Experimental details

Western blot analysis of HSPH1 using anti-HSPH1 antibody (M04168-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Caco-2 whole cell lysates Lane 2: human K562 whole cell lysates Lane 3: human A549 whole cell lysates Lane 4: human HepG2 whole cell lysates Lane 5: human PANC-1 whole cell lysates Lane 6: human SGC-7901 whole cell lysates After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-HSPH1 antigen affinity purified monoclonal antibody (Catalog # M04168-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for HSPH1 at approximately 105KD. The expected band size for HSPH1 is at 105KD.

Additional image