PA5-46980

antibody from Invitrogen Antibodies

Targeting: TREM2 TREM-2, Trem2a, Trem2b, Trem2c

Flow cytometry

Antibody data

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Validation data

Product number: PA5-46980 - Provider product page
Provider: Invitrogen Antibodies
Product name: Anti-TREM2 Polyclonal Antibody
Antibody type: Polyclonal
Antigen: Recombinant full-length protein
Description: In direct ELISAs, approximately 5% cross-reactivity with recombinant mouse (rm) TREM-2b is observed and less than 1% cross-reactivity with recombinant human (rh) TREM-1, rhTLT-1, rmTREM-L2, rmTREM-3 and rmTREM-4 is observed. Reconstitute at 0.2 mg/mL in sterile PBS.
Reactivity: Human
Host: Goat
Isotype: IgG
Vial size: 100 µg
Concentration: 0.2 mg/mL
Storage: -20° C, Avoid Freeze/Thaw Cycles

TREM2 protein structure - PA5-46980 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis from lysates of THP-1 human acute monocytic leukemia cell line. PVDF membrane was probed with 0.2 µg/mL of Goat Anti-human TREM-2 Antigen Affinity-purified Polyclonal Antibody (Product # PA5-46980) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody. A specific band was detected for TREM-2 at approximately 28 kDa (as indicated). This experiment was conducted under reducing conditions.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot was performed using TREM2 Polyclonal Antibody (Product # PA5-46980) and a 30 kDa band corresponding to TREM2 was observed in cell lines THP-1, THP-1 differentiated to Macrophage, THP-1 differentiated to Dendritic cells and condition media of THP-1, THP-1 differentiated to Macrophage, THP-1 differentiated to Dendritic cells; and was absent in RAW264.7 and condition media of Raw 264.7 along with two uncharacterized bands (*) 60 kDa and 12 kDa. Whole cell extracts (30 µg lysate) of THP-1 (Lane 1), THP-1 differentiated to Macrophage (Lane 2), THP-1 differentiated to Dendritic cells (Lane 3), RAW264.7 (Lane 4) of Fig a; and condition media of THP-1 (Lane 1), THP-1 differentiated to Macrophage (Lane 2), THP-1 differentiated to Dendritic cells (Lane 3), RAW264.7 (Lane 4) of Fig b were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0321BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # LC2001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (0.2 ug/ml) and detected by chemiluminescence with Rabbit anti-Goat IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27014, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).