Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Immunoprecipitation [1]
- Immunohistochemistry [4]
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Validation data
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- Product number
- NB100-77316 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-77316, RRID:AB_1084261
- Product name
- Rabbit Polyclonal CHIP/STUB1 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 ul
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C. Do not freeze.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
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- Experimental details
- Immunoprecipitation: CHIP/STUB1 Antibody [NB100-77316] - Detection of Human STUB1/CHIP by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-STUB1/CHIP antibody NB100-77316 used for IP at 3 mcg/mg lysate. STUB1/CHIP was also immunoprecipitated by rabbit anti-STUB1/CHIP antibody NB100-77315, which recognizes an upstream epitope. Detection: Chemiluminescence with an exposure time of 30 seconds.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: CHIP/STUB1 Antibody [NB100-77316] - Human prostate carcinoma. Affinity purified rabbit anti-STUB1/CHIP used at a dilution of 1:500.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: CHIP/STUB1 Antibody [NB100-77316] - Human breast carcinoma. Affinity purified rabbit anti-STUB1/CHIP used at a dilution of 1:500.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: CHIP/STUB1 Antibody [NB100-77316] - Mouse teratoma Affinity purified rabbit anti-STUB1/CHIP used at a dilution of 1:100.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: CHIP/STUB1 Antibody [NB100-77316] - Section of human breast carcinoma. Antibody: Affinity purified rabbit antiSTUB1/CHIP used at a dilution of 1:1,000 (1ug/ml). Detection: DAB