Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- PAB3640 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB3640, RRID:AB_1580622
- Product name
- SIGLEC8 polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic peptide of SIGLEC8.
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references Molecular characterization of a Siglec8 variant containing cytoplasmic tyrosine-based motifs, and mapping of the Siglec8 gene.
Identification of SAF-2, a novel siglec expressed on eosinophils, mast cells, and basophils.
Siglec-8. A novel eosinophil-specific member of the immunoglobulin superfamily.
Foussias G, Yousef GM, Diamandis EP
Biochemical and biophysical research communications 2000 Nov 30;278(3):775-81
Biochemical and biophysical research communications 2000 Nov 30;278(3):775-81
Identification of SAF-2, a novel siglec expressed on eosinophils, mast cells, and basophils.
Kikly KK, Bochner BS, Freeman SD, Tan KB, Gallagher KT, D'alessio KJ, Holmes SD, Abrahamson JA, Erickson-Miller CL, Murdock PR, Tachimoto H, Schleimer RP, White JR
The Journal of allergy and clinical immunology 2000 Jun;105(6 Pt 1):1093-100
The Journal of allergy and clinical immunology 2000 Jun;105(6 Pt 1):1093-100
Siglec-8. A novel eosinophil-specific member of the immunoglobulin superfamily.
Floyd H, Ni J, Cornish AL, Zeng Z, Liu D, Carter KC, Steel J, Crocker PR
The Journal of biological chemistry 2000 Jan 14;275(2):861-6
The Journal of biological chemistry 2000 Jan 14;275(2):861-6
No comments: Submit comment
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- The SIGLEC8 polyclonal antibody (Cat # PAB3640) is used in Western blot to detect SIGLEC8 in mouse liver tissue lysate (lnae 1) and in HL-60 cell lysate (lane 2) .
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded human cancer tissue reacted with the SIGLEC8 polyclonal antibody (Cat # PAB3640) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated. BC = breast carcinoma.
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
