MA5-27840

antibody from Invitrogen Antibodies

Targeting: ELP3 FLJ10422, KAT9

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-27840

Provider

Invitrogen Antibodies

Product name

ELP3 Monoclonal Antibody (GT11811)

Antibody type

Monoclonal

Antigen

Recombinant full-length protein

Description

Positive Control: HeLa, HeLa nuclear, 293T, A431, HeLa, HepG2

Antibody clone number

GT11811

Concentration

2.35 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of ELP3 in whole cell lysate using 30 µg of protein. Samples were separated with 7.5% SDS-PAGE and incubated with ELP3 monoclonal antibody (Product # MA5-27840) using a dilution of 1:500.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of ELP3 in HeLa cells using 30 µg of protein. Samples were separated with 7.5% SDS-PAGE and incubated with ELP3 monoclonal antibody (Product # MA5-27840) using a dilution of 1:1000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

ELP3 antibody detects ELP3 protein by western blot analysis. HeLa whole cell extracts and nuclear extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ELP3 antibody ELP3 Monoclonal Antibody (GT11811) (Product # MA5-27840) diluted by 1:1,000.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

ELP3 Monoclonal Antibody (GT11811) detects ELP3 protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: ELP3 protein stained by ELP3 Monoclonal Antibody (GT11811) (Product # MA5-27840) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 µm.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunoprecipitation of ELP3 was performed in 293T whole cell extracts using 5 µg of ELP3 Monoclonal Antibody (GT11811) (Product # MA5-27840). Samples were transferred to a membrane and probed with ELP3 Monoclonal Antibody (GT11811) as a primary antibody and an HRP-conjugated anti-Mouse IgG was used as a secondary antibody.