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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [3]
- Immunocytochemistry [1]
- Immunohistochemistry [5]
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- Product number
- PA5-63383 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Anti-RBPMS Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: PSTPLPNTVP QFIAREPYEL TVPALYPSSP EV Highest antigen sequence identity to the following orthologs: Mouse - 100%, Rat - 100%.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of RBPMS in human cell lines RT-4 and U-251MG using a RBPMS Polyclonal Antibody (Product # PA5-63383). Corresponding RBPMS RNA-seq data are presented for the same cell lines. Loading control: Anti-HSP90B1.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Knockdown of RBPMS was achieved by transfecting NTERA-2.cl.D1 cells with RBPMS specific siRNA (Silencer® select Product # s21729, s21728). Western blot analysis (Fig. a) was performed using whole cell extracts from the RBPMS knockdown cells (lane 3), non-specific scrambled siRNA transfected cells (lane 2) and untransfected cells (lane 1). The blot was probed with RBPMS Polyclonal Antibody (OTI4H6) (Product # PA5-63383, 1:1000 dilution) and Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution). Densitometric analysis of this western blot is shown in histogram (Fig. b). Decrease in signal upon siRNA mediated knock down confirms that antibody is specific to RBPMS.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot was performed using Anti-RBPMS Polyclonal Antibody (Product # PA5-63383) and a 24 kDa doublet corresponding to RBPMS were observed across cell line tissues tested. Whole cell extracts (30 µg lysate) of BeWo (Lane 1), NTERA-2.cl.D1 (Lane 2) and HeLa (Lane 3) were electrophoresed using Novex® NuPAGE® 12% Bis-Tris gel (Product # NP0342BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by XCell SureLock™ Mini-Cell and XCell II™ Blot Module (Product # EI0002). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of RBPMS in human cell line A549 shows positivity in cytoplasm & nucleus but excluded from the nucleoli. Samples were probed using a RBPMS Polyclonal Antibody (Product # PA5-63383).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of RBPMS in human endometrium and skin tissues using RBPMS Polyclonal Antibody (Product # PA5-63383). Corresponding RBPMS RNA-seq data are presented for the same tissues.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of RBPMS in human kidney using RBPMS Polyclonal Antibody (Product # PA5-63383) shows moderate to strong nuclear positivity in cells in tubules and glomeruli.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of RBPMS in human fallopian tube using RBPMS Polyclonal Antibody (Product # PA5-63383) shows moderate nuclear positivity in glandular cells.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of RBPMS in human skin using RBPMS Polyclonal Antibody (Product # PA5-63383) shows no positivity in squamous epithelial cells as expected.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of RBPMS in human endometrium using RBPMS Polyclonal Antibody (Product # PA5-63383) shows moderate nuclear positivity in glandular cells.
