PA5-27996

antibody from Invitrogen Antibodies

Targeting: BRAF BRAF1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-27996

Provider

Invitrogen Antibodies

Product name

BRAF35 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant protein fragment

Description

Recommended positive controls: 293T.

Concentration

0.95 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot using BRAF35 Polyclonal Antibody (Product # PA5-27996). Sample (30 µg whole cell lysate). A: 293T. 12% SDS PAGE. BRAF35 Polyclonal Antibody (Product # PA5-27996) diluted at 1:500.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

BRAF35 Polyclonal Antibody detects BRAF35 protein at nucleus by immunofluorescent analysis. Sample: A375 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: BRAF35 protein stained by BRAF35 Polyclonal Antibody (Product # PA5-27996) diluted at 1:500. Blue: Hoechst 33342 staining.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

BRAF35 Polyclonal Antibody immunoprecipitates BRAF35 protein in IP experiments. IP Sample: 293T whole cell lysate/extract A. 40 µg 293T whole cell lysate/extract B. Control with 2 µg of preimmune rabbit IgG C. Immunoprecipitation of BRAF35 protein by 2 µg of BRAF35 Polyclonal Antibody (Product # PA5-27996) 10% SDS-PAGE The immunoprecipitated BRAF35 protein was detected by BRAF35 Polyclonal Antibody (Product # PA5-27996) diluted at 1:1,000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

BRAF35 Polyclonal Antibody immunoprecipitates BRAF35 protein in IP experiments. IP Sample: 293T whole cell lysate/extract A. 40 µg 293T whole cell lysate/extract B. Control with 2 µg of preimmune rabbit IgG C. Immunoprecipitation of BRAF35 protein by 2 µg of BRAF35 Polyclonal Antibody (Product # PA5-27996) 10% SDS-PAGE The immunoprecipitated BRAF35 protein was detected by BRAF35 Polyclonal Antibody (Product # PA5-27996) diluted at 1:1,000.