PA5-93097

antibody from Invitrogen Antibodies

Targeting: HAGH GLO2, GLXII, HAGH1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-93097

Provider

Invitrogen Antibodies

Product name

HAGH Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Immunogen sequence: MKVEVLPALT DNYMYLVIDD ETKEAAIVDP VQPQKVVDAA RKHGVKLTTV LTTHHHWDHA GGNEKLVKLE SGLKVYGGDD RIGALTHKIT HLSTLQVGSL NVKCLATPCH TSGHICYFVS KPGGSEPPAV FTGDTLFVAG CGKFYEGTAD EMCKALLEVL GRLPPDTRVY CGHEYTINNL KFARHVEPGN AAIREKLAWA KEKYSIGEPT VPSTLAEEFT YNPFMRVREK TVQQHAGETD PVTTMRAVRR EKDQFKMPRD; Positive Samples: MCF7, HepG2, Mouse liver, Mouse kidney, Mouse heart, Rat liver, Rat kidney; Cellular Location: Cytoplasm, Mitochondrion matrix

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

1.27 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of extracts of various cell lines, using HAGH Polyclonal antibody (Product # PA5-93097) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Exposure time: 1s.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of HAGH in extracts of various cell lines using HAGH Polyclonal Antibody (Product # PA5-93097) at a dilution of 1:1000. A HRP Goat Anti-Rabbit IgG (H+L) secondary antibody was used at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of HAGH in extracts of various cell lines using HAGH Polyclonal Antibody (Product # PA5-93097) at a dilution of 1:1000. A HRP Goat Anti-Rabbit IgG (H+L) secondary antibody was used at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry-Immunofluorescence analysis of HAGH was performed in A549 cells using HAGH Polyclonal Antibody (Product # PA5-93097).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of HAGH in paraffin-embedded human liver cancer using HAGH Polyclonal Antibody (Product # PA5-93097) at a dilution of 1:100.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of HAGH in paraffin-embedded human esophageal cancer using HAGH Polyclonal Antibody (Product # PA5-93097) at a dilution of 1:100.