Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Flow cytometry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- PA1-772 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Anti-RAB3c Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- PA1-772 detects rab 3C from mouse and rat tissues and cells. PA1-772 has been successfully used in Western blot and immunoprecipitation procedures. By Western blot, this antibody detects a 22 kDa protein representing rab 3C from AtT20 cell extract. PA1-772 immunogen is a synthetic peptide corresponding to residues M(9) A S A Q D A R F G Q K D S S D Q N(26) C of rat RAB3C. This immunizing peptide (Cat. # PEP-106) is available for use in neutralization and control experiments.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 1 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot of rab 3C on AtT20 cell lysate using Product # PA1-772.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry analysis of RAB3C was done on SH-SY5Y cells. Cells were fixed with 70% ethanol for 10 minutes, permeabilized with 0.25% Triton™ X-100 for 20 minutes, and blocked with 5% BSA for 30 minutes at room temperature. Cells were labeled with RAB3C Rabbit Polyclonal Antibody (PA1772, red histogram) or with rabbit isotype control (yellow histogram) at 3-5 ug/million cells in 2.5% BSA. After incubation at room temperature for 2 hours, the cells were labeled with Alexa Fluor® 488 Goat Anti-Rabbit Secondary Antibody (A11008) at a dilution of 1:400 for 30 minutes at room temperature. The representative 10,000 cells were acquired and analyzed for each sample using an Attune® Acoustic Focusing Cytometer. The purple histogram represents unstained control cells and the green histogram represents no-primary-antibody control.