Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Flow cytometry [2]
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Validation data
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- Product number
- PA5-71808 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- IL1RL2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 400 µL
- Concentration
- 0.5 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of IL1RL2 in the following samples: Lane 1: HL-60 whole cell lysate, Lane 2: U-87 MG whole cell lysate, Lane 3: K562 whole cell lysate. 20 µg of protein per lane was used. Samples were first blocked with 5% NFDM/TBST, incubated with IL1RL2 polyclonal antibody (Product # PA5-71808) using a dilution of 1:8000 followed by a goat anti-rabbit IgG H&L (HRP) secondary antibody with a dilution of 1:10,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Overlay histogram showing K562 cells stained with PA5-71808 (green line). The cells were fixed with 2% paraformaldehyde (10 min). The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (PA5-71808, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed(OH191631) at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG (1?g/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry analysis of IL1RL2 in K562 cells (green line). The cells were fixed with 2% paraformaldhyde (10 min) and permeabilized with 90% methanol (10 min). The sample was then incubated with 2% BSA followed by a IL1RL2 polyclonal antibody (Product # PA5-71808) using a dilution of 1:25 for 60 min at 37°C. The secondary antibody used was goat-anti-rabbit IgG, DyLight 488 with a dilution of 1:200 for 40 min at 37°C. Isotype control antibody (blue line) was rabbit IgG (1 µg/1x10^6 cells) used under the same conditions with an acquisition of >10, 000 events.