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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- Immunohistochemistry [1]
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Validation data
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- Product number
- PA5-50607 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CD297 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- The antibody detects endogenous levels of total CD297 / ART4 protein.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 2 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Art4 was performed by loading (from left to right): Mouse kidney, liver, heart and brain tissue lysates (40µg) on to a 10% SDS-PAGE gel. Proteins were transferred to a membrane and the membrane was probed with a Art4 antibody (Product # PA5-50607) at a 1/667 dilution for 2 minutes, followed by a secondary antibody at 1/8000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Art4 was performed by loading (from left to right): Mouse kidney, liver, heart and brain tissue lysates (40µg) on to a 10% SDS-PAGE gel. Proteins were transferred to a membrane and the membrane was probed with a Art4 antibody (Product # PA5-50607) at a 1/667 dilution for 2 minutes, followed by a secondary antibody at 1/8000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Art4 was performed by loading (from left to right): Mouse kidney, liver, heart and brain tissue lysates (40µg) on to a 10% SDS-PAGE gel. Proteins were transferred to a membrane and the membrane was probed with a Art4 antibody (Product # PA5-50607) at a 1/667 dilution for 2 minutes, followed by a secondary antibody at 1/8000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of CD297 in paraffin embedded Human cervical cancer tissue using CD297 Polyclonal Antibody (Product # PA5-50607) at a 1:30 dilution.
