Antibody data
- Antibody Data
- Antigen structure
- References [4]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [5]
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Validation data
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- Product number
- NBP1-84071 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-84071, RRID:AB_11014413
- Product name
- Rabbit Polyclonal DMRT1 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Specificity of human DMRT1 antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references Protein expression of the transcription factors DMRT1, TCLF5, and OCT4 in selected germ cell neoplasms of the testis.
The human testis-specific proteome defined by transcriptomics and antibody-based profiling.
Fibroblast growth factor receptor 3 is highly expressed in rarely dividing human type A spermatogonia.
Differential marker protein expression specifies rarefaction zone-containing human Adark spermatogonia.
Roth LM, Michal M, Michal M Jr, Cheng L
Human pathology 2018 Dec;82:68-75
Human pathology 2018 Dec;82:68-75
The human testis-specific proteome defined by transcriptomics and antibody-based profiling.
Djureinovic D, Fagerberg L, Hallström B, Danielsson A, Lindskog C, Uhlén M, Pontén F
Molecular human reproduction 2014 Jun;20(6):476-88
Molecular human reproduction 2014 Jun;20(6):476-88
Fibroblast growth factor receptor 3 is highly expressed in rarely dividing human type A spermatogonia.
von Kopylow K, Staege H, Schulze W, Will H, Kirchhoff C
Histochemistry and cell biology 2012 Nov;138(5):759-72
Histochemistry and cell biology 2012 Nov;138(5):759-72
Differential marker protein expression specifies rarefaction zone-containing human Adark spermatogonia.
von Kopylow K, Staege H, Spiess AN, Schulze W, Will H, Primig M, Kirchhoff C
Reproduction (Cambridge, England) 2012 Jan 1;143(1):45-57
Reproduction (Cambridge, England) 2012 Jan 1;143(1):45-57
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: DMRT1 Antibody [NBP1-84071] - Analysis in control (vector only transfected HEK293T lysate) and DMRT1 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: DMRT1 Antibody [NBP1-84071] - Staining of human fallopian tube shows low expression as expected.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: DMRT1 Antibody [NBP1-84071] - Staining of human testis shows high expression.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: DMRT1 Antibody [NBP1-84071] - Analysis in human testis and fallopian tube tissues. Corresponding DMRT1 RNA-seq data are presented for the same tissues.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: DMRT1 Antibody [NBP1-84071] - Staining of human fallopian tube shows no nuclear positivity in glandular cells as expected.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: DMRT1 Antibody [NBP1-84071] - Staining of human liver shows no positivity in hepatocytes as expected.