Antibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Flow cytometry [1]
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Validation data
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- Product number
- 14-2449-82 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CD244 Monoclonal Antibody (eBioPP35 (PP35)), eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: The eBioPP35 monoclonal antibody reacts with human CD244, also known as 2B4, p38, NK-cell activation-inducing ligand, signaling lymphocyte activation molecule family member 4, and natural killer cell receptor 2B4. Human CD244 is a 38 kD protein expressed on NK cells, a subset of CD8+ T cells, gammadelta T cells, monocytes, basophils, mast cells, and eosinophils. Binding of CD244 to its ligand, CD48, results in NK cell activation. Costaining experiments with eBioC1.7 suggest that both antibodies recognize different epitopes. Applications Reported: This eBioPP35 (PP35) antibody has been reported for use in flow cytometric analysis, immunoprecipitation, and immunoblotting (WB). Purity: Greater than 90%, as determined by SDS-PAGE. Aggregation: Less than 10%, as determined by HPLC. Filtration: 0.2 µm post-manufacturing filtered.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- eBioPP35 (PP35)
- Vial size
- 100 µg
- Concentration
- 0.5 mg/mL
- Storage
- 4° C
Submitted references Differential Inhibitory Receptor Expression on T Cells Delineates Functional Capacities in Chronic Viral Infection.
IL-7Rαlow memory CD8+ T cells are significantly elevated in patients with systemic lupus erythematosus.
Novel surface molecules involved in human NK cell activation and triggering of the lytic machinery.
Teigler JE, Zelinskyy G, Eller MA, Bolton DL, Marovich M, Gordon AD, Alrubayyi A, Alter G, Robb ML, Martin JN, Deeks SG, Michael NL, Dittmer U, Streeck H
Journal of virology 2017 Dec 1;91(23)
Journal of virology 2017 Dec 1;91(23)
IL-7Rαlow memory CD8+ T cells are significantly elevated in patients with systemic lupus erythematosus.
Kim JS, Cho BA, Sim JH, Shah K, Woo CM, Lee EB, Lee DS, Kang JS, Lee WJ, Park CG, Craft J, Kang I, Kim HR
Rheumatology (Oxford, England) 2012 Sep;51(9):1587-94
Rheumatology (Oxford, England) 2012 Sep;51(9):1587-94
Novel surface molecules involved in human NK cell activation and triggering of the lytic machinery.
Moretta A, Bottino C, Tripodi G, Vitale M, Pende D, Morelli L, Augugliaro R, Barbaresi M, Ciccone E, Millo R
International journal of cancer. Supplement = Journal international du cancer. Supplement 1992;7:6-10
International journal of cancer. Supplement = Journal international du cancer. Supplement 1992;7:6-10
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Supportive validation
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- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Staining of normal human peripheral blood cells with Anti-Human CD8a APC (Product # 17-0088-42) and 0.25 µg of Mouse IgG1 kappa Isotype Control Purified (Product # 14-4714-82) (left) or 0.25 µg of Anti-Human CD244 Purified (right). Cells in the lymphocyte gate were used for analysis.