MA5-27719

antibody from Invitrogen Antibodies

Targeting: ATP7B WND

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Recommended by provider)

Immunohistochemistry (Recommended by provider)

Antibody Data

Product number

MA5-27719

Provider

Invitrogen Antibodies

Product name

ATP7B Monoclonal Antibody (S62-29)

Antibody type

Monoclonal

Antigen

Synthetic peptide

Reactivity

Human, Mouse, Rat

Host

Mouse

Isotype

IgG

Antibody clone number

S62-29

Vial size

100 µg

Concentration

1 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of ATP7B in rat brain membrane with 20 µg of sample. The sample was blocked with 2% BSA and 2% skim milk in TBST, incubated with ATP7B monoclonal antibody (Product # MA5-27719) using a dilution of 1:1000 (16 hours at 4°C), followed by Goat Anti-Mouse HRP (1 hour at RT) at a dilution of 1:100 and ECL development (6 min at RT). Samples were arranged as follows: Lane 1: Molecular Weight Ladder (MW), Lane 2: Rat Brain membrane cell lysate.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of ATP7B in mouse NIH 3T3 (mouse fibroblast cell line). Sample was fixed with 4% formaldehyde (15 min at RT), incubated with ATP7B monoclonal antibody (Product # MA5-27719) using a dilution of 1:100 (1 hour at RT), and followed by Goat Anti-Mouse 488, Phalloidin Texas Red and DAPI secondary antibody at a dilution of 1:200, 1:1000 (60 min at RT) and 1:5000 (5 min at RT). Images are shown as follows: (A) DAPI (blue) nuclear stain, B) Phalloidin Texas Red F-Actin stain, C) Copper Transporting ATPase 2 Antibody, D) Merged image. Magnification: 60x.