H00003108-M01

antibody from Abnova Corporation

Targeting: HLA-DMA D6S222E, RING6

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

H00003108-M01

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00003108-M01, RRID:AB_425480

Product name

HLA-DMA monoclonal antibody (M01), clone 3F12-F11

Antibody type

Monoclonal

Description

Mouse monoclonal antibody raised against a full length recombinant HLA-DMA.

Antigen sequence

MGHEQNQGAALLQMLPLLWLLPHSWAVPEAPTPMW
PDDLQNHTFLHTVYCQDGSPSVGLSEAYDEDQLFF
FDFSQNTRVPRLPEFADWAQEQGDAPAILFDKEFC
EWMIQQIGPKLDGKIPVSRGFPIAEVFTLKPLEFG
KPNTLVCFVSNLFPPMLTVNWQHHSVPVEGFGPTF
VSAVDGLSFQAFSYLNFTPEPSDIFSCIVTHEIDR
YTAIAYWVPRNALPSDLLENVLCGVAFGLGVLGII
VGIVLIIYFRKPCSGD


Isotype

IgG

Antibody clone number

3F12-F11

Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

HLA-DMA monoclonal antibody (M01), clone 3F12-F11 Western Blot analysis of HLA-DMA expression in U-2 OS ( Cat # L022V1 ).

ELISA

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Detection limit for recombinant GST tagged HLA-DMA is approximately 1ng/ml as a capture antibody.

Validation comment

Sandwich ELISA (Recombinant protein)

Protocol

Protocol

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunoperoxidase of monoclonal antibody to HLA-DMA on formalin-fixed paraffin-embedded human malignant lymphoma, diffuse large B tissue. [antibody concentration 3 ug/ml]

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Protocol

Protocol