AP1475a

antibody from Abgent

Targeting: STAP1 BRDG1, STAP-1

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

AP1475a

Provider

Abgent

Proper citation

Abgent Cat#AP1475a, RRID:AB_1208464

Product name

BRDG 1 Antibody (N-term F56)

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

Purified Rabbit Polyclonal Antibody (Pab)

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Vial size

400 µl

Concentration

2 mg/ml

Storage

Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

Abgent (provider)

Main image

Experimental details

Western blot analysis of anti-STAP1 Antibody (N-term) (F56) (Cat.#AP1475a) in mouse kidney tissue lysates (35ug/lane). STAP(arrow) was detected using the purified Pab.

Primary Ab dilution

1:1000

Supportive validation

Submitted by

Abgent (provider)

Main image

Experimental details

Western blot analysis of STAP1 (arrow) using rabbit polyclonal STAP1 Antibody (N-term) (F56) (Cat.#AP1475a). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the STAP1 gene.

Primary Ab dilution

1:1000

Immunohistochemistry

Supportive validation

Submitted by

Abgent (provider)

Main image

Experimental details

"Formalin-fixed and paraffin-embedded human prostate carcinoma tissue reacted with STAP1 antibody (N-term)(F56) (Cat.#AP1475a), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated."

Primary Ab dilution

1:10~50