orb18977

antibody from Biorbyt

Targeting: CD274 B7-H, B7-H1, B7H1, PD-L1, PDCD1LG1, PDL1

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Immunocytochemistry (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Data presented on provider website)

Antibody Data

Product number

orb18977

Provider

Biorbyt

Proper citation

Biorbyt Cat#orb18977, RRID:AB_10751131

Product name

CD274 antibody

Antibody type

Polyclonal

Description

Goat polyclonal antibody to CD274

Reactivity

Human

Host

Goat

Conjugate

Unconjugated

Vial size

100 μg

Storage

Aliquot and store at -20°C. Minimize freezing and thawing.

Western blot

Supportive validation

Submitted by

Biorbyt (provider)

Main image

Experimental details

orb18977 staining (0.3ug/ml) of Human Heart lysate (RIPA buffer, 30ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

Immunohistochemistry

Supportive validation

Submitted by

Biorbyt (provider)

Main image

Experimental details

orb18977 (4ug/ml) staining of paraffin embedded Human Skeletal Muscle. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.