Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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Validation data
Reference
Comment
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- Product number
- orb18977 - Provider product page
- Provider
- Biorbyt
- Proper citation
- Biorbyt Cat#orb18977, RRID:AB_10751131
- Product name
- CD274 antibody
- Antibody type
- Polyclonal
- Description
- Goat polyclonal antibody to CD274
- Reactivity
- Human
- Host
- Goat
- Conjugate
- Unconjugated
- Vial size
- 100 μg
- Storage
- Aliquot and store at -20°C. Minimize freezing and thawing.
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Supportive validation
- Submitted by
- Biorbyt (provider)
- Main image
- Experimental details
- orb18977 staining (0.3ug/ml) of Human Heart lysate (RIPA buffer, 30ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
Supportive validation
- Submitted by
- Biorbyt (provider)
- Main image
- Experimental details
- orb18977 (4ug/ml) staining of paraffin embedded Human Skeletal Muscle. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.