R20329

antibody from NSJ Bioreagents

Targeting: CD274 B7-H, B7-H1, B7H1, PD-L1, PDCD1LG1, PDL1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

R20329

Provider

NSJ Bioreagents

Product name

Recombinant PD-L1 Antibody / B7-H1 / CD274 / C-Terminal

Antibody type

Monoclonal

Description

Protein A purified from animal origin-free supernatant

Reactivity

Human

Host

Rabbit

Conjugate

Unconjugated

Antibody clone number

RM311

Vial size

100 µl

Storage

Store the recombinant PD-L1 antibody at -20°C.

Western blot

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot testing of human H1975 (lung cancer line), DU145 (prostate cancer line) and LNCaP (prostate cancer line) cell lysate with recombinant PD-L1 antibody at 1:100 dilution. Predicted molecular weight: 34-70 kDa depending on glycosylation level.

Immunocytochemistry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IF / ICC testing of human H1975 cells (lung cancer line) with recombinant PD-L1 antibody at 1:100 dilution (red). Nuclei have been labeled with DAPI (blue).

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

ICC testing of formalin fixed and paraffin embedded human H1975 cells (lung cancer line) with recombinant PD-L1 antibody at 1:300 dilution. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.

Immunohistochemistry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC testing of formalin fixed and paraffin embedded human tonsil tissue with recombinant PD-L1 antibody at 1:300 dilution. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.