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LearnAF165
antibody from Novus Biologicals
Targeting: ICOSLG
B7-H2, B7h, B7H2, B7RP-1, B7RP1, CD275, GL50, ICOS-L, ICOSL, KIAA0653
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [1]
- Flow cytometry [1]
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- Product number
- AF165 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Goat Polyclonal B7-H2/ICOSLG Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects human B7-H2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 1% cross-reactivity with recombinant mouse B7-H2 and recombinant human B7-H1 is observed.
- Reactivity
- Human
- Host
- Goat
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Submitted references 12-O-tetradecanoyl phorbol 13-acetate induces the expression of B7-DC, -H1, -H2, and -H3 in K562 cells.
The immunomodulatory proteins B7-DC, B7-H2, and B7-H3 are differentially expressed across gestation in the human placenta.
Jang BC, Park YK, Choi IH, Kim SP, Hwang JB, Baek WK, Suh MH, Mun KC, Suh SI
International journal of oncology 2007 Dec;31(6):1439-47
International journal of oncology 2007 Dec;31(6):1439-47
The immunomodulatory proteins B7-DC, B7-H2, and B7-H3 are differentially expressed across gestation in the human placenta.
Petroff MG, Kharatyan E, Torry DS, Holets L
The American journal of pathology 2005 Aug;167(2):465-73
The American journal of pathology 2005 Aug;167(2):465-73
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of B7-H2 by Western Blot. Western blot shows lysate of U937 human histiocytic lymphoma cell line. PVDF membrane was probed with 2 µg/mL of Goat Anti-Human B7-H2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF165) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for B7-H2 at approximately 75 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of B7-H2 in U937 Human Cell Line by Flow Cytometry. U937 human histiocytic lymphoma cell line was stained with Goat Anti-Human B7-H2 Antigen Affinity-purified Polyclonal Anti-body (Catalog # AF165, filled histogram) or isotype control antibody (Catalog # AB-108-C, open histogram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107).
