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ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [20]
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- Validations
- Western blot [3]
- Immunohistochemistry [1]
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- Product number
- NB100-1965 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-1965, RRID:AB_10002123
- Product name
- Rabbit Polyclonal Calnexin Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human, Mouse, Rat, Bovine, Chicken/Avian, Drosophila, Guinea Pig, Porcine, Rabbit, Sheep, Xenopus, Zebrafish
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Concentration
- 1 mg/ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references Enhanced ER-associated degradation of HMG CoA reductase causes embryonic lethality associated with Ubiad1 deficiency.
Macrophage miR-34a Is a Key Regulator of Cholesterol Efflux and Atherosclerosis.
Rigorous characterization of urinary extracellular vesicles (uEVs) in the low centrifugation pellet - a neglected source for uEVs.
Hepatocyte-Specific Expression of Human Carboxylesterase 1 Attenuates Diet-Induced Steatohepatitis and Hyperlipidemia in Mice.
Extracellular vesicles from endometriosis patients are characterized by a unique miRNA-lncRNA signature.
Exosomal Release of L-Plastin by Breast Cancer Cells Facilitates Metastatic Bone Osteolysis.
Yak-milk-derived exosomes promote proliferation of intestinal epithelial cells in an hypoxic environment.
Schnyder corneal dystrophy-associated UBIAD1 inhibits ER-associated degradation of HMG CoA reductase in mice.
Two Patched molecules engage distinct sites on Hedgehog yielding a signaling-competent complex.
Structures of human Patched and its complex with native palmitoylated sonic hedgehog.
Mitochondrial ASncmtRNA-1 and ASncmtRNA-2 as potent targets to inhibit tumor growth and metastasis in the RenCa murine renal adenocarcinoma model.
Farnesoid X receptor activation increases reverse cholesterol transport by modulating bile acid composition and cholesterol absorption in mice.
Inactivation of Tm6sf2, a Gene Defective in Fatty Liver Disease, Impairs Lipidation but Not Secretion of Very Low Density Lipoproteins.
Characterization of Regulatory Extracellular Vesicles from Osteoclasts.
Mutations in the Histone Modifier PRDM6 Are Associated with Isolated Nonsyndromic Patent Ductus Arteriosus.
Scap is required for sterol synthesis and crypt growth in intestinal mucosa.
Disease mutations in CMP-sialic acid transporter SLC35A1 result in abnormal α-dystroglycan O-mannosylation, independent from sialic acid.
Characterization of Ca(2+) signaling in the external yolk syncytial layer during the late blastula and early gastrula periods of zebrafish development.
Importance of uncharged polar residues and proline in the proximal two-thirds (Pro107-Ser128) of the highly conserved region of mouse ileal Na+-dependent bile acid transporter, Slc10a2, in transport activity and cellular expression.
O-glycosylation modulates integrin and FGF signalling by influencing the secretion of basement membrane components.
Jo Y, Kim SS, Garland K, Fuentes I, DiCarlo LM, Ellis JL, Fu X, Booth SL, Evers BM, DeBose-Boyd RA
eLife 2020 Mar 2;9
eLife 2020 Mar 2;9
Macrophage miR-34a Is a Key Regulator of Cholesterol Efflux and Atherosclerosis.
Xu Y, Xu Y, Zhu Y, Sun H, Juguilon C, Li F, Fan D, Yin L, Zhang Y
Molecular therapy : the journal of the American Society of Gene Therapy 2020 Jan 8;28(1):202-216
Molecular therapy : the journal of the American Society of Gene Therapy 2020 Jan 8;28(1):202-216
Rigorous characterization of urinary extracellular vesicles (uEVs) in the low centrifugation pellet - a neglected source for uEVs.
Musante L, Bontha SV, La Salvia S, Fernandez-Piñeros A, Lannigan J, Le TH, Mas V, Erdbrügger U
Scientific reports 2020 Feb 28;10(1):3701
Scientific reports 2020 Feb 28;10(1):3701
Hepatocyte-Specific Expression of Human Carboxylesterase 1 Attenuates Diet-Induced Steatohepatitis and Hyperlipidemia in Mice.
Xu Y, Zhu Y, Bawa FC, Hu S, Pan X, Yin L, Zhang Y
Hepatology communications 2020 Apr;4(4):527-539
Hepatology communications 2020 Apr;4(4):527-539
Extracellular vesicles from endometriosis patients are characterized by a unique miRNA-lncRNA signature.
Khalaj K, Miller JE, Lingegowda H, Fazleabas AT, Young SL, Lessey BA, Koti M, Tayade C
JCI insight 2019 Sep 19;4(18)
JCI insight 2019 Sep 19;4(18)
Exosomal Release of L-Plastin by Breast Cancer Cells Facilitates Metastatic Bone Osteolysis.
Tiedemann K, Sadvakassova G, Mikolajewicz N, Juhas M, Sabirova Z, Tabariès S, Gettemans J, Siegel PM, Komarova SV
Translational oncology 2019 Mar;12(3):462-474
Translational oncology 2019 Mar;12(3):462-474
Yak-milk-derived exosomes promote proliferation of intestinal epithelial cells in an hypoxic environment.
Gao HN, Guo HY, Zhang H, Xie XL, Wen PC, Ren FZ
Journal of dairy science 2019 Feb;102(2):985-996
Journal of dairy science 2019 Feb;102(2):985-996
Schnyder corneal dystrophy-associated UBIAD1 inhibits ER-associated degradation of HMG CoA reductase in mice.
Jo Y, Hamilton JS, Hwang S, Garland K, Smith GA, Su S, Fuentes I, Neelam S, Thompson BM, McDonald JG, DeBose-Boyd RA
eLife 2019 Feb 20;8
eLife 2019 Feb 20;8
Two Patched molecules engage distinct sites on Hedgehog yielding a signaling-competent complex.
Qi X, Schmiege P, Coutavas E, Li X
Science (New York, N.Y.) 2018 Oct 5;362(6410)
Science (New York, N.Y.) 2018 Oct 5;362(6410)
Structures of human Patched and its complex with native palmitoylated sonic hedgehog.
Qi X, Schmiege P, Coutavas E, Wang J, Li X
Nature 2018 Aug;560(7716):128-132
Nature 2018 Aug;560(7716):128-132
Mitochondrial ASncmtRNA-1 and ASncmtRNA-2 as potent targets to inhibit tumor growth and metastasis in the RenCa murine renal adenocarcinoma model.
Borgna V, Villegas J, Burzio VA, Belmar S, Araya M, Jeldes E, Lobos-González L, Silva V, Villota C, Oliveira-Cruz L, Lopez C, Socias T, Castillo O, Burzio LO
Oncotarget 2017 Jul 4;8(27):43692-43708
Oncotarget 2017 Jul 4;8(27):43692-43708
Farnesoid X receptor activation increases reverse cholesterol transport by modulating bile acid composition and cholesterol absorption in mice.
Xu Y, Li F, Zalzala M, Xu J, Gonzalez FJ, Adorini L, Lee YK, Yin L, Zhang Y
Hepatology (Baltimore, Md.) 2016 Oct;64(4):1072-85
Hepatology (Baltimore, Md.) 2016 Oct;64(4):1072-85
Inactivation of Tm6sf2, a Gene Defective in Fatty Liver Disease, Impairs Lipidation but Not Secretion of Very Low Density Lipoproteins.
Smagris E, Gilyard S, BasuRay S, Cohen JC, Hobbs HH
The Journal of biological chemistry 2016 May 13;291(20):10659-76
The Journal of biological chemistry 2016 May 13;291(20):10659-76
Characterization of Regulatory Extracellular Vesicles from Osteoclasts.
Huynh N, VonMoss L, Smith D, Rahman I, Felemban MF, Zuo J, Rody WJ Jr, McHugh KP, Holliday LS
Journal of dental research 2016 Jun;95(6):673-9
Journal of dental research 2016 Jun;95(6):673-9
Mutations in the Histone Modifier PRDM6 Are Associated with Isolated Nonsyndromic Patent Ductus Arteriosus.
Li N, Subrahmanyan L, Smith E, Yu X, Zaidi S, Choi M, Mane S, Nelson-Williams C, Behjati M, Kazemi M, Hashemi M, Fathzadeh M, Narayanan A, Tian L, Montazeri F, Mani M, Begleiter ML, Coon BG, Lynch HT, Olson EN, Zhao H, Ruland J, Lifton RP, Mani A
American journal of human genetics 2016 Jun 2;98(6):1082-1091
American journal of human genetics 2016 Jun 2;98(6):1082-1091
Scap is required for sterol synthesis and crypt growth in intestinal mucosa.
McFarlane MR, Cantoria MJ, Linden AG, January BA, Liang G, Engelking LJ
Journal of lipid research 2015 Aug;56(8):1560-71
Journal of lipid research 2015 Aug;56(8):1560-71
Disease mutations in CMP-sialic acid transporter SLC35A1 result in abnormal α-dystroglycan O-mannosylation, independent from sialic acid.
Riemersma M, Sandrock J, Boltje TJ, Büll C, Heise T, Ashikov A, Adema GJ, van Bokhoven H, Lefeber DJ
Human molecular genetics 2015 Apr 15;24(8):2241-6
Human molecular genetics 2015 Apr 15;24(8):2241-6
Characterization of Ca(2+) signaling in the external yolk syncytial layer during the late blastula and early gastrula periods of zebrafish development.
Yuen MY, Webb SE, Chan CM, Thisse B, Thisse C, Miller AL
Biochimica et biophysica acta 2013 Jul;1833(7):1641-56
Biochimica et biophysica acta 2013 Jul;1833(7):1641-56
Importance of uncharged polar residues and proline in the proximal two-thirds (Pro107-Ser128) of the highly conserved region of mouse ileal Na+-dependent bile acid transporter, Slc10a2, in transport activity and cellular expression.
Saeki T, Sato K, Ito S, Ikeda K, Kanamoto R
BMC physiology 2013 Feb 4;13:4
BMC physiology 2013 Feb 4;13:4
O-glycosylation modulates integrin and FGF signalling by influencing the secretion of basement membrane components.
Tian E, Hoffman MP, Ten Hagen KG
Nature communications 2012 May 29;3:869
Nature communications 2012 May 29;3:869
No comments: Submit comment
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Calnexin Antibody [NB100-1965] - WB analysis of CANX in cell lysates as noted.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Simple Western: Calnexin Antibody [NB100-1965] - Simple Western lane view shows a specific band for Calnexin in 1.0 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Calnexin Antibody [NB100-1965] - A dilution of 1/2000 used in TBS with 0.05% Tween-20. Bands visualized by ECL using goat anti-rabbit HRP at 1:2000.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry: Calnexin Antibody [NB100-1965] - IHC analysis of CANX in mouse prostate using DAB with hematoxylin counterstain.
