Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [3]
- Immunohistochemistry [7]
- Flow cytometry [1]
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Validation data
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- Product number
- MA5-32545 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- PDHA1 Recombinant Rabbit Monoclonal Antibody (JF996-0)
- Antibody type
- Monoclonal
- Antigen
- Synthetic peptide
- Description
- Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Antibody clone number
- JF996-0
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Abnormal lipid droplets accumulation induced cognitive deficits in obstructive sleep apnea syndrome mice via JNK/SREBP/ACC pathway but not through PDP1/PDC pathway.
Li D, Xu N, Hou Y, Ren W, Zhang N, Wang X, Sun Y, Lu W, Qu G, Yu Y, Lv C, Han F
Molecular medicine (Cambridge, Mass.) 2022 Jan 14;28(1):3
Molecular medicine (Cambridge, Mass.) 2022 Jan 14;28(1):3
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of PDHA1 in different lysates using a Monoclonal antibody (Product #MA5-32545) at a dilution of 1:1,000. Positive control: Lane 1: 293T, Lane 2: A431, Lane 3: Mouse heart.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemical analysis of PDHA1 in Hela cells using a PDHA1 Monoclonal antibody (Product # MA5-32545) as seen in red. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemical analysis of PDHA1 in HepG2 cells using a PDHA1 Monoclonal antibody (Product # MA5-32545) as seen in red. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemical analysis of PDHA1 in SH-SY-5Y cells using a PDHA1 Monoclonal antibody (Product # MA5-32545) as seen in red. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of PDHA1 of paraffin-embedded Human lung cancer tissue using a PDHA1 Monoclonal antibody (Product #MA5-32545). Counter stained with hematoxylin.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of PDHA1 of paraffin-embedded Mouse stomach tissue using a PDHA1 Monoclonal antibody (Product #MA5-32545). Counter stained with hematoxylin.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of PDHA1 of paraffin-embedded Human breast carcinoma tissue using a PDHA1 Monoclonal antibody (Product #MA5-32545). Counter stained with hematoxylin.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of PDHA1 of paraffin-embedded Human kidney tissue using a PDHA1 Monoclonal antibody (Product #MA5-32545). Counter stained with hematoxylin.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of PDHA1 of paraffin-embedded Mouse skeletal muscle tissue using a PDHA1 Monoclonal antibody (Product #MA5-32545). Counter stained with hematoxylin.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of PDHA1 of paraffin-embedded Human lung cancer tissue using a PDHA1 Monoclonal antibody (Product #MA5-32545). Counter stained with hematoxylin.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of PDHA1 of paraffin-embedded Mouse colon tissue using a PDHA1 Monoclonal antibody (Product #MA5-32545). Counter stained with hematoxylin.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow Cytometric analysis of PDHA1 in Hela cells using a PDHA1 Monoclonal Antibody (Product # MA5-32545) at a dilution of 1:50, as seen in red compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.