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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunoprecipitation [1]
- Chromatin Immunoprecipitation [1]
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- Product number
- GTX129240 - Provider product page
- Provider
- GeneTex
- Product name
- MAFK antibody
- Antibody type
- Polyclonal
- Reactivity
- Human
- Host
- Rabbit
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Supportive validation
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- GeneTex (provider)
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- Experimental details
- MAFK antibody detects MAFK protein by western blot analysis.A. 30 £gg Jurkat whole cell lysate/extract 15 % SDS-PAGEMAFK antibody (GTX129240) dilution: 1:500
Supportive validation
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- GeneTex (provider)
- Main image
- Experimental details
- MAFK antibody immunoprecipitates MAFK protein in IP experiments.IP samples: Jurkat whole cell extractA. Control with 4 £gg of preimmune Rabbit IgGB. Immunoprecipitation of MAFK protein by 4 £gg MAFK antibody (GTX129240)15 % SDS-PAGEThe immunoprecipitated MAFK protein was detected by MAFK antibody (GTX129240) diluted at 1:500.[EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Cross-linked ChIP was performed with HepG2 chromatin extract treated with LPS (10 ng/ml for 1 h) and 5 £gg of either normal rabbit IgG or anti-MAFK antibody. The precipitated DNA was detected by PCR with primer set targeting to IL-8 or GAPDH .
