PA5-85947

antibody from Invitrogen Antibodies

Targeting: NRAS N-ras

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Recommended by provider)

Antibody Data

Product number

PA5-85947

Provider

Invitrogen Antibodies

Product name

Pan Ras Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

1 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of Pan Ras in different lysates using a Polyclonal antibody (Product #PA5-85947) at a dilution of 1:1000. Positive control: Lane 1: PC12, Lane 2: MCF-7, Lane 3: 293T, Lane 4: Mouse brain, Lane 5: Mouse intestine, Lane 6: Rat brain.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemical analysis of Pan Ras in HCT116 cells using a Pan Ras Polyclonal antibody (Product # PA5-85947) as seen in green. Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemical analysis of Pan Ras in HepG2 cells using a Pan Ras Polyclonal antibody (Product # PA5-85947) as seen in green. Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemical analysis of Pan Ras in HCT116 cells using a Pan Ras Polyclonal antibody (Product # PA5-85947) as seen in green. Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemical analysis of Pan Ras in HepG2 cells using a Pan Ras Polyclonal antibody (Product # PA5-85947) as seen in green. Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemical analysis of Pan Ras in Hela cells using a Pan Ras Polyclonal antibody (Product # PA5-85947) as seen in green. Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemical analysis of Pan Ras in MCF-7 cells using a Pan Ras Polyclonal antibody (Product # PA5-85947) as seen in green. Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of Pan Ras of paraffin-embedded Mouse kidney tissue using a Pan-Ras Polyclonal antibody (Product #PA5-85947). Counter stained with hematoxylin.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of Pan Ras of paraffin-embedded Mouse small intestine tissue using a Pan-Ras Polyclonal antibody (Product #PA5-85947). Counter stained with hematoxylin.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of Pan Ras of paraffin-embedded Mouse kidney tissue using a Pan-Ras Polyclonal antibody (Product #PA5-85947). Counter stained with hematoxylin.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of Pan Ras of paraffin-embedded rat small intestine tissue using a Pan-Ras Polyclonal antibody (Product #PA5-85947). Counter stained with hematoxylin.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of Pan Ras of paraffin-embedded Human liver cancer tissue using a Pan-Ras Polyclonal antibody (Product #PA5-85947). Counter stained with hematoxylin.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of Pan Ras of paraffin-embedded Human kidney muscle tissue using a Pan-Ras Polyclonal antibody (Product #PA5-85947). Counter stained with hematoxylin.