Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
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Validation data
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- Product number
- 601201 - Provider product page
- Provider
- BioLegend
- Proper citation
- BioLegend Cat#601201, RRID:AB_2715902
- Product name
- Purified anti-TDRD3
- Antibody type
- Monoclonal
- Reactivity
- Human
- Host
- Rat
- Conjugate
- Unconjugated
- Antibody clone number
- W16174A
- Vial size
- 25 µg
- Concentration
- 0.5 mg/ml
- Storage
- 2°C-8°C
- Handling
- Ambient RT
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Supportive validation
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- BioLegend (provider)
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- Experimental details
- Total cell lysate (15 µg protein) from U87-MG, HeLa and NIH3T3 cells were resolved by electrophoresis (4-12% Bis-Tris gel), transferred to nitrocellulose, and probed with 1:250 (2 µg/mL) diluted purified anti-TDRD3 antibody (clone W16174A) or competitor’s antibody used at manufactures recommended concentration (upper). Proteins were visualized by chemiluminescence detection using 1:3000 diluted HRP rat-IgG secondary antibody conjugated to HRP (clone Poly4054) for clone W16174A or 1:3000 diluted donkey anti-rabbit IgG Antibody conjugated to HRP (clone Poly4054) for competitor’s antibody (upper). 1:2000 dilution of Direct-Blot™ HRP anti-β-actin antibody (clone 2F1-1) was used as a loading control (lower). Lane M: MW ladder.
- Conjugate
- Unconjugated