703456

antibody from Invitrogen Antibodies

Targeting: GRSF1

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

703456

Provider

Invitrogen Antibodies

Product name

GRSF1 Recombinant Rabbit Monoclonal Antibody (11H24L16)

Antibody type

Monoclonal

Antigen

Other

Description

This antibody is predicted to react with Monkey, Bovine, Pig, Mouse.

Antibody clone number

11H24L16

Concentration

0.5 mg/mL

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

For immunofluorescence analysis, MCF7 cells were fixed and permeabilized for detection of endogenous GRSF1 using Anti-GRSF1 Recombinant Rabbit Monoclonal Antibody (Product # 703456, 1:100 dilution) and labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034, 1:2000). Panel a) shows representative cells that were stained for detection and localization of GRSF1 protein (green), Panel b) is stained for nuclei (blue) using ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). Panel c) represents cytoskeletal F-actin staining using Rhodamine Phalloidin (Product # R415, 1:300). Panel d) is a composite image of Panels a, b and c clearly demonstrating mitochondrial localization of GRSF1. Panel e) represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Knockdown of GRSF1 was achieved by transfecting HeLa cells with specific siRNA (Silencer® select Product # s6234, s6233). Immunofluorescence analysis was performed on HeLa cells (untransfected, panel a-d), transfected with GRSF1 specific siRNA (panel i-l) or non-specific scrambled siRNA (panels e-h). Cells were fixed, permeabilized, and labeled with Anti-GRSF1 Recombinant Rabbit Monoclonal Antibody (Product # 703456, 1:100 dilution), followed by Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034, 1:2000). Nuclei (blue) were stained using ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962), and Rhodamine Phalloidin (Product # R415, 1:300) was used for cytoskeletal F-actin (red) staining. Significant reduction of signal was observed upon siRNA mediated knockdown (panel i-l) confirming specificity of the antibody to GRSF1 (green). The images were captured at 60X magnification.