ABIN2616358

antibody from antibodies-online

Targeting: ANKRD17 FLJ22206, GTAR, KIAA0697, MASK2, NY-BR-16

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Immunocytochemistry (Supportive data in Antibodypedia)

Chromatin Immunoprecipitation (Supportive data in Antibodypedia)

Antibody Data

Product number

ABIN2616358

Provider

antibodies-online

Product name

anti-Ankyrin Repeat Domain 17 (ANKRD17) (AA 2501-2604) antibody

Antibody type

Monoclonal

Description

Affinity purified

Reactivity

Human

Host

Mouse

Epitope

AA 2501-2604

Isotype

IgG

Vial size

100 μg

Storage

May be stored at 4°C for short-term only. Aliquot to avoid freeze-thaw cycles. Store at -20°C. Aliquots are stable for at least 1 year.

Handling

Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

antibodies-online (provider)

Main image

Experimental details

Histone H3 monomethyl Lys4 antibody tested by Western blot. HeLa acid extract probed with Histone H3 monomethyl Lys4 antibody (1:5,000 dilution).

Supportive validation

Submitted by

antibodies-online (provider)

Main image

Experimental details

Histone H3 monomethyl Lys4 antibody tested by dot blot analysis. Dot blot analysis was used to confirm the specificity of Histone H3 monomethyl Lys4 antibody for monomethyl-Lys4 histone H3. Methylated peptides corresponding to the immunogen and related peptides were spotted onto PVDF and probed with the antibody at 1:10,000. The amount of peptide (picomoles) spotted is indicated next to each row. Lane 1: Unmodified lysine 4 peptide. Lane 2: Monomethyl lysine 4 peptide. Lane 3: Dimethyl lysine 4 peptide. Lane 4: Trimethyl lysine 4 peptide. Lane 5: Monomethyl lysine 4 peptide #2. Lane 6: Unmodified peptide corresponding to amino acids 6-22 of human histone H3. Lane 7: Monomethyl lysine 9 peptide. Lane 8: Dimethyl lysine 9 peptide. Lane 9: Trimethyl lysine 9 peptide. Lane 10: Dimethyl lysine 9 peptide #2. Lane 11: Unmodified lysine 27 peptide. Lane 12: Monomethyl lysine 27 peptide. Lane 13: Dimethyl lysine 27 peptide. Lane 14: Trimethyl lysine 27 peptide. Lane 15: Monomethyl lysine 27 peptide #2. Lane 16: Dimethyl lysine 14 peptide.

Immunocytochemistry

Supportive validation

Submitted by

antibodies-online (provider)

Main image

Experimental details

Histone H3 monomethyl Lys4 antibody (pAb) tested by immunofluorescence. HeLa cells stained with Histone H3 monomethyl Lys4 antibody (1:1,000). Top: Histone H3 monomethyl Lys4 antibody. Middle: DAPI. Bottom: merge.

Chromatin Immunoprecipitation

Supportive validation

Submitted by

antibodies-online (provider)

Main image

Experimental details

Histone H3 monomethyl Lys4 antibody tested by ChIP-Seq. ChIP was performed using the ChIP-IT® High Sensitivity Kit (Cat. No. 53040) with chromatin from human prostate cancer cells (3 million). ChIP DNA was sequenced on the Illumina GA II and 22 million sequence tags were mapped to identify H3K4me1 binding sites. The image shows H3K4me1 binding across a 600 Kb region on chromosome 13.

Supportive validation

Submitted by

antibodies-online (provider)

Main image

Experimental details

Histone H3 monomethyl Lys4 antibody specificity tested by peptide array analysis. Peptide array analysis was used to confirm the specificity of this antibody for its intended modification. Histone H3 monomethyl Lys4 antibody was applied at a dilution of 1:5,000 to Active Motif's MODified? Histone Peptide Array (Catalog No. 13001). The arrays were scanned with ArrayAnalysis Software 7 and the results plotted. Specificity data is shown for the most reactive peptides and those related to the immunogen. Recognition of the H3 monomethyl Lys4 peptide is blocked by Thr3 phosphorylation, Arg2 methylation and a citrulline at position 2. Array Data File