AF1529

antibody from Novus Biologicals

Targeting: HAVCR2 CD366, FLJ14428, Tim-3, TIM3, TIMD3

Western blot (Supportive data in Antibodypedia)

Flow cytometry (Data presented on provider website)

Antibody Data

Product number

AF1529

Provider

Novus Biologicals

Product name

Goat Polyclonal TIM-3 Antibody

Antibody type

Polyclonal

Description

Antigen Affinity-purified. Detects mouse TIM-3 in direct ELISAs and Western blots. In direct ELISAs, approximately 10% cross-reactivity with recombinant human TIM-3 is observed and less than 5% cross-reactivity with recombinant mouse (rm) TIM-1, rmTIM-2, rmTIM-4, rmTIM-5, rmTIM-6, and rmTIM-7 is observed.

Reactivity

Mouse

Host

Goat

Conjugate

Unconjugated

Isotype

IgG

Vial size

100 ug

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.

References

Submitted references

Transcriptomic Hallmarks of Tumor Plasticity and Stromal Interactions in Brain Metastasis.

Wingrove E, Liu ZZ, Patel KD, Arnal-Estapé A, Cai WL, Melnick MA, Politi K, Monteiro C, Zhu L, Valiente M, Kluger HM, Chiang VL, Nguyen DX
Cell reports 2019 Apr 23;27(4):1277-1292.e7

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The HIF-1/glial TIM-3 axis controls inflammation-associated brain damage under hypoxia.

Koh HS, Chang CY, Jeon SB, Yoon HJ, Ahn YH, Kim HS, Kim IH, Jeon SH, Johnson RS, Park EJ
Nature communications 2015 Mar 20;6:6340

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TIM-1 and TIM-3 enhancement of Th2 cytokine production by mast cells.

Nakae S, Iikura M, Suto H, Akiba H, Umetsu DT, Dekruyff RH, Saito H, Galli SJ
Blood 2007 Oct 1;110(7):2565-8

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Detection of Mouse TIM-3 by Western Blot. Detection of Mouse TIM-3 by Western Blot.