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- Antigen structure
- References [15]
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- Immunohistochemistry [1]
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- Product number
- AF2365 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Goat Polyclonal TIM-3 Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects human TIM-3 in direct ELISAs and Western blots. In direct ELISAs, approximately 5% cross-reactivity with recombinant mouse (rm) TIM-3 is observed and less than 1% cross-reactivity with recombinant human TIM-1, rmTIM-1, and rmTIM-2 is observed.
- Reactivity
- Human
- Host
- Goat
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Submitted references Microtubule-Driven Stress Granule Dynamics Regulate Inhibitory Immune Checkpoint Expression in T Cells.
Galectin-9 as a Predictive Marker for the Onset of Immune-Related Adverse Effects Associated with Anti-CCR4 MoAb Therapy in Patients with Adult T Cell Leukemia.
Secretion of IFN-γ Associated with Galectin-9 Production by Pleural Fluid Cells from a Patient with Extrapulmonary Tuberculosis.
TIM-1 Promotes Hepatitis C Virus Cell Attachment and Infection.
Frontline Science: Tim-3-mediated dysfunctional engulfment of apoptotic cells in SLE.
Tim-3 is a Marker of Plasmacytoid Dendritic Cell Dysfunction during HIV Infection and Is Associated with the Recruitment of IRF7 and p85 into Lysosomes and with the Submembrane Displacement of TLR9.
Comprehensive analyses of tumor immunity: implications for cancer immunotherapy.
Soluble T cell immunoglobulin mucin domain 3 is shed from CD8+ T cells by the sheddase ADAM10, is increased in plasma during untreated HIV infection, and correlates with HIV disease progression.
Characterizing functional domains for TIM-mediated enveloped virus entry.
T cell Ig and mucin domain-containing protein 3 is recruited to the immune synapse, disrupts stable synapse formation, and associates with receptor phosphatases.
Tim-3 expression defines regulatory T cells in human tumors.
Decreased galectin-9 and increased Tim-3 expression are related to poor prognosis in gastric cancer.
Tim-3 negatively regulates IL-12 expression by monocytes in HCV infection.
Dysregulation of TIM-3-galectin-9 pathway in the cystic fibrosis airways.
Beneficial effect of galectin 9 on rheumatoid arthritis by induction of apoptosis of synovial fibroblasts.
Franchini DM, Lanvin O, Tosolini M, Patras de Campaigno E, Cammas A, Péricart S, Scarlata CM, Lebras M, Rossi C, Ligat L, Pont F, Arimondo PB, Laurent C, Ayyoub M, Despas F, Lapeyre-Mestre M, Millevoi S, Fournié JJ
Cell reports 2019 Jan 2;26(1):94-107.e7
Cell reports 2019 Jan 2;26(1):94-107.e7
Galectin-9 as a Predictive Marker for the Onset of Immune-Related Adverse Effects Associated with Anti-CCR4 MoAb Therapy in Patients with Adult T Cell Leukemia.
Mohammed TO, Chagan-Yasutan H, Ashino Y, Nakayama W, Takahashi Y, Shimomura T, Fujimoto T, Watanabe Y, Niki T, Suzushima H, Hattori T
The Tohoku journal of experimental medicine 2017 Mar;241(3):201-208
The Tohoku journal of experimental medicine 2017 Mar;241(3):201-208
Secretion of IFN-γ Associated with Galectin-9 Production by Pleural Fluid Cells from a Patient with Extrapulmonary Tuberculosis.
Zhao J, Shiratori B, Chagan-Yasutan H, Matsumoto M, Niki T, Tanaka M, Takahashi Y, Usami O, Ashino Y, Hattori T
International journal of molecular sciences 2017 Jun 28;18(7)
International journal of molecular sciences 2017 Jun 28;18(7)
TIM-1 Promotes Hepatitis C Virus Cell Attachment and Infection.
Wang J, Qiao L, Hou Z, Luo G
Journal of virology 2017 Jan 15;91(2)
Journal of virology 2017 Jan 15;91(2)
Frontline Science: Tim-3-mediated dysfunctional engulfment of apoptotic cells in SLE.
Zhao D, Guo M, Liu B, Lin Q, Xie T, Zhang Q, Jia X, Shu Q, Liang X, Gao L, Ma C
Journal of leukocyte biology 2017 Dec;102(6):1313-1322
Journal of leukocyte biology 2017 Dec;102(6):1313-1322
Tim-3 is a Marker of Plasmacytoid Dendritic Cell Dysfunction during HIV Infection and Is Associated with the Recruitment of IRF7 and p85 into Lysosomes and with the Submembrane Displacement of TLR9.
Schwartz JA, Clayton KL, Mujib S, Zhang H, Rahman AK, Liu J, Yue FY, Benko E, Kovacs C, Ostrowski MA
Journal of immunology (Baltimore, Md. : 1950) 2017 Apr 15;198(8):3181-3194
Journal of immunology (Baltimore, Md. : 1950) 2017 Apr 15;198(8):3181-3194
Comprehensive analyses of tumor immunity: implications for cancer immunotherapy.
Li B, Severson E, Pignon JC, Zhao H, Li T, Novak J, Jiang P, Shen H, Aster JC, Rodig S, Signoretti S, Liu JS, Liu XS
Genome biology 2016 Aug 22;17(1):174
Genome biology 2016 Aug 22;17(1):174
Soluble T cell immunoglobulin mucin domain 3 is shed from CD8+ T cells by the sheddase ADAM10, is increased in plasma during untreated HIV infection, and correlates with HIV disease progression.
Clayton KL, Douglas-Vail MB, Nur-ur Rahman AK, Medcalf KE, Xie IY, Chew GM, Tandon R, Lanteri MC, Norris PJ, Deeks SG, Ndhlovu LC, Ostrowski MA
Journal of virology 2015 Apr;89(7):3723-36
Journal of virology 2015 Apr;89(7):3723-36
Characterizing functional domains for TIM-mediated enveloped virus entry.
Moller-Tank S, Albritton LM, Rennert PD, Maury W
Journal of virology 2014 Jun;88(12):6702-13
Journal of virology 2014 Jun;88(12):6702-13
T cell Ig and mucin domain-containing protein 3 is recruited to the immune synapse, disrupts stable synapse formation, and associates with receptor phosphatases.
Clayton KL, Haaland MS, Douglas-Vail MB, Mujib S, Chew GM, Ndhlovu LC, Ostrowski MA
Journal of immunology (Baltimore, Md. : 1950) 2014 Jan 15;192(2):782-91
Journal of immunology (Baltimore, Md. : 1950) 2014 Jan 15;192(2):782-91
Tim-3 expression defines regulatory T cells in human tumors.
Yan J, Zhang Y, Zhang JP, Liang J, Li L, Zheng L
PloS one 2013;8(3):e58006
PloS one 2013;8(3):e58006
Decreased galectin-9 and increased Tim-3 expression are related to poor prognosis in gastric cancer.
Jiang J, Jin MS, Kong F, Cao D, Ma HX, Jia Z, Wang YP, Suo J, Cao X
PloS one 2013;8(12):e81799
PloS one 2013;8(12):e81799
Tim-3 negatively regulates IL-12 expression by monocytes in HCV infection.
Zhang Y, Ma CJ, Wang JM, Ji XJ, Wu XY, Jia ZS, Moorman JP, Yao ZQ
PloS one 2011;6(5):e19664
PloS one 2011;6(5):e19664
Dysregulation of TIM-3-galectin-9 pathway in the cystic fibrosis airways.
Vega-Carrascal I, Reeves EP, Niki T, Arikawa T, McNally P, O'Neill SJ, Hirashima M, McElvaney NG
Journal of immunology (Baltimore, Md. : 1950) 2011 Mar 1;186(5):2897-909
Journal of immunology (Baltimore, Md. : 1950) 2011 Mar 1;186(5):2897-909
Beneficial effect of galectin 9 on rheumatoid arthritis by induction of apoptosis of synovial fibroblasts.
Seki M, Sakata KM, Oomizu S, Arikawa T, Sakata A, Ueno M, Nobumoto A, Niki T, Saita N, Ito K, Dai SY, Katoh S, Nishi N, Tsukano M, Ishikawa K, Yamauchi A, Kuchroo V, Hirashima M
Arthritis and rheumatism 2007 Dec;56(12):3968-76
Arthritis and rheumatism 2007 Dec;56(12):3968-76
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- TIM-3 in Human Tonsil. TIM-3 in Human Tonsil.
