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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Western blot [3]
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- Product number
- PA1-739 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- SCAMP1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- PA1-739 detects secretory carrier membrane protein 1 (SCAMP 1) from human, mouse and rat samples.
- Concentration
- 1 mg/mL
Submitted references Tissue expression of the vesicle protein pantophysin.
Tissue expression of the vesicle protein pantophysin.
Windoffer R, Borchert-Stuhlträger M, Haass NK, Thomas S, Hergt M, Bulitta CJ, Leube RE
Cell and tissue research 1999 Jun;296(3):499-510
Cell and tissue research 1999 Jun;296(3):499-510
Tissue expression of the vesicle protein pantophysin.
Windoffer R, Borchert-Stuhlträger M, Haass NK, Thomas S, Hergt M, Bulitta CJ, Leube RE
Cell and tissue research 1999 Jun;296(3):499-510
Cell and tissue research 1999 Jun;296(3):499-510
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot of SCAMP 1 on rat brain extract using Product # PA1-739.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis was performed on membrane extracts (30 µglysate) of U-87 MG (Lane 1), Neuro-2a (Lane 2), SH-SY5Y (Lane 3), SK-N-AS (Lane 4), T98G (Lane5), IMR-32 (Lane 6), and tissue extract (30 µglysate) of rat brain (Lane 7). The blots were probed with Rabbit Anti-SCAMP1 Polyclonal Antibody (Product # PA1-739, 2 µg/mL) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/mL, 1:4000 dilution). A 36 kDa band corresponding to SCAMP1 was observed across the cell lines and tissues tested. Known quantity of protein samples were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0321BOX), XCell SureLock™ Electrophoresis System (Product # EI0002) and Novex® Sharp Pre-Stained Protein Standard (Product # LC5800). Resolved proteins were then transferred onto a nitrocellulose membrane with iBlot® 2 Dry Blotting System (Product # IB21001). The membrane was probed with the relevant primary and secondary Antibody following blocking with 5 % skimmed milk. Chemiluminescent detection was performed using Pierce™ ECL Western Blotting Substrate (Product # 32106).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis was performed on membrane extracts (30 µglysate) of U-87 MG (Lane 1), Neuro-2a (Lane 2), SH-SY5Y (Lane 3), SK-N-AS (Lane 4), T98G (Lane5), IMR-32 (Lane 6), and tissue extract (30 µglysate) of rat brain (Lane 7). The blots were probed with Rabbit Anti-SCAMP1 Polyclonal Antibody (Product # PA1-739, 2 µg/mL) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/mL, 1:4000 dilution). A 36 kDa band corresponding to SCAMP1 was observed across the cell lines and tissues tested. Known quantity of protein samples were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0321BOX), XCell SureLock™ Electrophoresis System (Product # EI0002) and Novex® Sharp Pre-Stained Protein Standard (Product # LC5800). Resolved proteins were then transferred onto a nitrocellulose membrane with iBlot® 2 Dry Blotting System (Product # IB21001). The membrane was probed with the relevant primary and secondary Antibody following blocking with 5 % skimmed milk. Chemiluminescent detection was performed using Pierce™ ECL Western Blotting Substrate (Product # 32106).
