Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [5]
- Immunocytochemistry [1]
- Immunoprecipitation [1]
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Validation data
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- Product number
- GTX128974 - Provider product page
- Provider
- GeneTex
- Product name
- PER1 antibody
- Antibody type
- Polyclonal
- Reactivity
- Human, Rat
- Host
- Rabbit
Submitted references The important tumor suppressor role of PER1 in regulating the cyclin-CDK-CKI network in SCC15 human oral squamous cell carcinoma cells.
Fu XJ, Li HX, Yang K, Chen D, Tang H
OncoTargets and therapy 2016;9:2237-45
OncoTargets and therapy 2016;9:2237-45
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Supportive validation
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- GeneTex (provider)
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- Experimental details
- PER1 antibody detects PER1 protein by western blot analysis.A. 30 μg Rat2 whole cell lysate/extract5 % SDS-PAGEPER1 antibody (GTX128974) dilution: 1:1000
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- GeneTex (provider)
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- Experimental details
- PER1 antibody detects PER1 protein by western blot analysis.A. 30 £gg 293T whole cell lysate/extract B. 30 £gg HeLa whole cell lysate/extract C. 30 £gg HepG2 whole cell lysate/extract5 % SDS-PAGEPER1 antibody (GTX128974) dilution: 1:1000
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- GeneTex (provider)
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- Experimental details
- PER1 antibody detects PER1 protein by western blot analysis.A. 30 £gg drosophila lysate/extract5 % SDS-PAGEPER1 antibody (GTX128974) dilution: 1:500
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- GeneTex (provider)
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- Experimental details
- Various tissue extracts (50 ?g) were separated by 5% SDS-PAGE, and the membrane was blotted with PER1 antibody (GTX128974) diluted at 1:1000.
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- GeneTex (provider)
- Main image
- Experimental details
- Various whole cell extracts (30 ?g) were separated by 5% SDS-PAGE, and the membrane was blotted with PER1 antibody (GTX128974) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Supportive validation
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- GeneTex (provider)
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- Experimental details
- PER1 antibody detects PER1 protein at nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: PER1 protein stained by PER1 antibody (GTX128974) diluted at 1:1000.Blue: Hoechst 33342 staining.
Supportive validation
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- Experimental details
- PER1 antibody immunoprecipitates PER1 protein in IP experiments.IP samples: HeLa nuclear extractA. Control with 4 £gg of preimmune Rabbit IgGB. Immunoprecipitation of PER1 protein by 4 £gg PER1 antibody (GTX128974)5 % SDS-PAGEThe immunoprecipitated PER1 protein was detected by PER1 antibody (GTX128974) diluted at 1:500.[EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]