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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
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- Product number
- RQ6667 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- OS9 Antibody / Amplified in Osteosarcoma 9
- Antibody type
- Polyclonal
- Description
- Antigen affinity purified
- Reactivity
- Human, Rat
- Host
- Rabbit
- Vial size
- 100 µg
- Concentration
- 0.5mg/ml if reconstituted with 0.2ml sterile DI water
- Storage
- After reconstitution, the OS9 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of 1) human HeLa, 2) human 293T, 3) human HL60, 4) rat pancreas and 5) mouse pancreas tissue lysate with OS9 antibody. Expected molecular weight: 61-76 kDa (multiple isoforms) but may be observed at higher molecular weights due to glycosylation. Isoform OS-9-1 is commonly observed at 76-97 kDa and isoform OS-9-2 is commonly observed at 69-83 kDa.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Immunofluorescent staining of FFPE human SiHa cells with OS9 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
