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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
- Blocking/Neutralizing [1]
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- Product number
- AF1068 - Provider product page
- Provider
- R&D Systems
- Product name
- Mouse Epiregulin Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects mouse Epiregulin in direct ELISAs and Western blots. In direct ELISAs, approximately 40% cross-reactivity with recombinant human Epiregulin is observed. In Western blots, recombinant mouse Proepiregulin and mature Epiregulin is detected.
- Reactivity
- Mouse
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
Q61521- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references The luteinizing hormone receptor-activated extracellularly regulated kinase-1/2 cascade stimulates epiregulin release from granulosa cells.
Andric N, Ascoli M
Endocrinology 2008 Nov;149(11):5549-56
Endocrinology 2008 Nov;149(11):5549-56
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Mouse Epiregulin by Western Blot. Western blot shows lysates of P19 mouse embryonal carcinoma cell line. PVDF Membrane was probed with 1 µg/mL of Goat Anti-Mouse Epiregulin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1068) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Proepiregulin at approximately 28 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Cell Proliferation Induced by Epiregulin and Neutralization by Mouse Epiregulin Antibody. Recombinant Mouse Epiregulin (Catalog # 1068-EP) stimulates proliferation in the Balb/3T3 mouse embryonic fibroblast cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse Epiregulin (3 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse Epiregulin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1068). The ND50 is typically 0.3-1 µg/mL.
