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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- TA328749 - Provider product page
- Provider
- OriGene
- Product name
- Rabbit Polyclonal Anti-TRPM7
- Antibody type
- Polyclonal
- Description
- Rabbit Polyclonal Anti-TRPM7
- Host
- Rabbit
- Conjugate
- Unconjugated
- Epitope
- TRPM7
- Antibody clone number
- NULL
- Vial size
- 200 µl
- Concentration
- NULL
Submitted references Aberrant over-expression of TRPM7 ion channels in pancreatic cancer: required for cancer cell invasion and implicated in tumor growth and metastasis.
Yee NS, Kazi AA, Li Q, Yang Z, Berg A, Yee RK
Biology open 2015 Mar 13;4(4):507-14
Biology open 2015 Mar 13;4(4):507-14
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot analysis of GH3 cell line (lanes 1 and 2) and mouse brain (lanes 3 and 4) lysates: 1,3. Anti-TRPM7 antibody, (1:200). 2,4. Anti-TRPM7 antibody, preincubated with the control peptide antigen.
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Expression of TRPM7 in GH3 cell lines. Immunocytochemical staining of GH3 cells with Anti-TRPM7 antibody, (1:100), followed by goat anti-rabbit-AlexaFluor-550 secondary antibody (A). B. Nuclear staining of GH3 cells with the cell-permeable dye Hoechst 33342. C. Live intact GH3 cells.
- Validation comment
- IF
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Expression of TRPM7 in rat pituitary gland. Immunohistochemical staining of rat hypophysis paraffin embedded transversal sections using Anti-TRPM7 antibody , (1:50). TRPM7 is stained in both the posterior part or neurohypophysis (N) and the anterior part or adenohypophysis (A). Note that stain is highly specific for andocrine cells in the adenohypophysis. Color reaction was obtained with SuperPicture HRP-conjugated polymer (Zymed) followed by DAB. Hematoxilin is used as the Counterstain.
- Validation comment
- IHC
