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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Flow cytometry [1]
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- Product number
- MAB3495 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Mouse Monoclonal LRIG3 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human LRIG3 in direct ELISAs and Western blots. Detects mouse LRIG3 in Western blots. Mouse LRIG3 detection in Flow Cytometry was not tested. In direct ELISAs, no cross-reactivity with recombinant human LRIG1 is observed.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of Human and Mouse LRIG3 by Western Blot. Western blot shows lysates of KATO-III human gastric carcinoma cell line, HEK293T human embryonic kidney cell line, and B16-F1 mouse melanoma cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human LRIG3 Monoclonal Antibody (Catalog # MAB3495) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for LRIG3 at approximately 160 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of LRIG3 in KATO-III Human Cell Line by Flow Cytometry. KATO-III human gastric carcinoma cell line was stained with Mouse Anti-Human LRIG3 Monoclonal Antibody (Catalog # MAB3495, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B).
