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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- PA5-27227 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Cystatin F Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: A549, MCF-7.
- Concentration
- 0.98 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of cystatin F using 30 µg of A) A549 and B) MCF-7 lysate. Samples were loaded onto a 12% SDS-PAGE gel and probed with a cystatin F polyclonal antibody (Product # PA5-27227) at a dilution of 1:1000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded CL1-5 xenograft, using cystatin F (Product # PA5-27227) antibody at 1:500 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
