A302-648A-T

antibody from Invitrogen Antibodies

Targeting: PYGO1

Western blot (Recommended by provider)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

A302-648A-T

Provider

Invitrogen Antibodies

Product name

Pygo1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

10 µL

Concentration

1 mg/mL

Storage

4° C

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human Pygo1 by immunohistochemistry. Sample: FFPE section of human colon carcinoma. Antibody: Affinity purified rabbit anti- Pygo1 (Cat. No. A302-648A Lot1) used at a dilution of 1:1,000 (1µg/ml). Detection: DAB.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human Pygo1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-Pygo1 antibody A302-648A used for IP at 3 µg/mg lysate. Pygo1 was also immunoprecipitated by rabbit anti-Pygo1 antibody A302-649A, which recognizes a downstream epitope. For blotting immunoprecipitated Pygo1, A302-649A was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.