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- Antibody Data
- Antigen structure
- References [10]
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- Immunohistochemistry [1]
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- Product number
- AF3743 - Provider product page
- Provider
- R&D Systems
- Product name
- Human Ephrin-A5 Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects human Ephrin-A5 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant mouse (rm) Ephrin-A1, rmEphrin-A2, recombinant human (rh) Ephrin-A3, rhEphrin-A4, rhEphrin-B3, rmEphrin-A4, rmEphrin-B1 and rmEphrin-B2 is observed.
- Reactivity
- Human
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
P52803- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references EphA4 may contribute to microvessel remodeling in the hippocampal CA1 and CA3 areas in a mouse model of temporal lobe epilepsy.
Role of synaptic plasticity and EphA5-ephrinA5 interaction within the ventromedial hypothalamus in response to recurrent hypoglycemia.
β3GnT2 null mice exhibit defective accessory olfactory bulb innervation.
EphA5-EphrinA5 interactions within the ventromedial hypothalamus influence counterregulatory hormone release and local glutamine/glutamate balance during hypoglycemia.
EphrinA5 acts as a tumor suppressor in glioma by negative regulation of epidermal growth factor receptor.
p75(NTR) mediates ephrin-A reverse signaling required for axon repulsion and mapping.
Meltrin beta/ADAM19 interacting with EphA4 in developing neural cells participates in formation of the neuromuscular junction.
BIG-2 mediates olfactory axon convergence to target glomeruli.
EphA-Ephrin-A-mediated beta cell communication regulates insulin secretion from pancreatic islets.
Adam meets Eph: an ADAM substrate recognition module acts as a molecular switch for ephrin cleavage in trans.
Feng L, Shu Y, Wu Q, Liu T, Long H, Yang H, Li Y, Xiao B
Molecular medicine reports 2017 Jan;15(1):37-46
Molecular medicine reports 2017 Jan;15(1):37-46
Role of synaptic plasticity and EphA5-ephrinA5 interaction within the ventromedial hypothalamus in response to recurrent hypoglycemia.
Szepietowska B, Horvath TL, Sherwin RS
Diabetes 2014 Mar;63(3):1140-7
Diabetes 2014 Mar;63(3):1140-7
β3GnT2 null mice exhibit defective accessory olfactory bulb innervation.
Henion TR, Madany PA, Faden AA, Schwarting GA
Molecular and cellular neurosciences 2013 Jan;52:73-86
Molecular and cellular neurosciences 2013 Jan;52:73-86
EphA5-EphrinA5 interactions within the ventromedial hypothalamus influence counterregulatory hormone release and local glutamine/glutamate balance during hypoglycemia.
Szepietowska B, Zhu W, Czyzyk J, Eid T, Sherwin RS
Diabetes 2013 Apr;62(4):1282-8
Diabetes 2013 Apr;62(4):1282-8
EphrinA5 acts as a tumor suppressor in glioma by negative regulation of epidermal growth factor receptor.
Li JJ, Liu DP, Liu GT, Xie D
Oncogene 2009 Apr 16;28(15):1759-68
Oncogene 2009 Apr 16;28(15):1759-68
p75(NTR) mediates ephrin-A reverse signaling required for axon repulsion and mapping.
Lim YS, McLaughlin T, Sung TC, Santiago A, Lee KF, O'Leary DD
Neuron 2008 Sep 11;59(5):746-58
Neuron 2008 Sep 11;59(5):746-58
Meltrin beta/ADAM19 interacting with EphA4 in developing neural cells participates in formation of the neuromuscular junction.
Yumoto N, Wakatsuki S, Kurisaki T, Hara Y, Osumi N, Frisén J, Sehara-Fujisawa A
PloS one 2008 Oct 2;3(10):e3322
PloS one 2008 Oct 2;3(10):e3322
BIG-2 mediates olfactory axon convergence to target glomeruli.
Kaneko-Goto T, Yoshihara S, Miyazaki H, Yoshihara Y
Neuron 2008 Mar 27;57(6):834-46
Neuron 2008 Mar 27;57(6):834-46
EphA-Ephrin-A-mediated beta cell communication regulates insulin secretion from pancreatic islets.
Konstantinova I, Nikolova G, Ohara-Imaizumi M, Meda P, Kucera T, Zarbalis K, Wurst W, Nagamatsu S, Lammert E
Cell 2007 Apr 20;129(2):359-70
Cell 2007 Apr 20;129(2):359-70
Adam meets Eph: an ADAM substrate recognition module acts as a molecular switch for ephrin cleavage in trans.
Janes PW, Saha N, Barton WA, Kolev MV, Wimmer-Kleikamp SH, Nievergall E, Blobel CP, Himanen JP, Lackmann M, Nikolov DB
Cell 2005 Oct 21;123(2):291-304
Cell 2005 Oct 21;123(2):291-304
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Supportive validation
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- R&D Systems (provider)
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- Experimental details
- Ephrin-A5 in Human Melanoma. Ephrin-A5 was detected in immersion fixed paraffin-embedded sections of human melanoma using Goat Anti-Human Ephrin-A5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3743) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
