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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Western blot [1]
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- Product number
- ABIN498027 - Provider product page
- Provider
- antibodies-online
- Product name
- anti-Like-Glycosyltransferase (LARGE) (Internal Region), (AA 421-433) antibody
- Antibody type
- Polyclonal
- Antigen
- Peptide with sequence from the internal region of the protein sequence according to NP_004728.1. Genename: LARGE
- Description
- Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide.
- Reactivity
- Human, Bovine, Canine
- Host
- Goat
- Antigen sequence
C-SEADVNSENL QKQ- Epitope
- Internal Region,AA 421-433
- Vial size
- 0.1 mg
- Concentration
- 0.5 mg/mL
- Storage
- Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
- Handling
- Avoid repeated freezing and thawing.
Submitted references Loss of alpha-dystroglycan laminin binding in epithelium-derived cancers is caused by silencing of LARGE.
de Bernabé DB, Inamori K, Yoshida-Moriguchi T, Weydert CJ, Harper HA, Willer T, Henry MD, Campbell KP
The Journal of biological chemistry 2009 Apr 24;284(17):11279-84
The Journal of biological chemistry 2009 Apr 24;284(17):11279-84
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Supportive validation
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- antibodies-online (provider)
- Main image
- Experimental details
- AP20114PU-N LARGE antibody staining of Human Kidney lysate at 1 μg/ml (RIPA buffer, 35 μg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
