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LearnH00009750-M02
antibody from Abnova Corporation
Targeting: RIPOR2
C6orf32, DIFF48, FAM65B, KIAA0386, MYONAP
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- ELISA [1]
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Validation data
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- Product number
- H00009750-M02 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#H00009750-M02, RRID:AB_463931
- Product name
- C6orf32 monoclonal antibody (M02), clone 2F6-1A11
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody raised against a full length recombinant C6orf32.
- Antigen sequence
MLVGSQSFSPGGPNGIIRSQSFAGFSGLQERRSRC
NSFIENSSALKKPQAKLKKMHNLGHKNNNPPKEPQ
PKRVEEVYRALKNGLDEYLEVHQTELDKLTAQLKD
MKRNSRLGVLYDLDKQIKTIERYMRRLEFHISKVD
ELYEAYCIQRRLQDGASKMKQAFATSPASKAARES
LTEINRSFKEYTENMCTIEVELENLLGEFSIKMKG
LAGFARLCPGDQYEIFMKYGRQRWKLKGKIEVNGK
QSWDGEETVFLPLIVGFISIKVTELKGLATHILVG
SVTCETKELFAARPQVVAVDINDLGTIKLNLEITW
YPFDVEDMTASSGAGNKAAALQRRMSMYSQGTPET
PTFKDHSFFSNLPDDIFENGKAAEEKMPLSLSFSD
LPNGDCALTSHSTGSPSNSTNPEITITPAEFNLSS
LASQNEGMDDTSSASSRNSLGEGQEPKSHLKEEDP
EEPRKPASAPSEACRRQSSGAGAEHLFLENDVAEA
LLQESEEASELKPVELDTSEGNITKQLVKRLTSAE
VPMATDRLLSEGSVGGESEGCRSFLDGSLEDAFNG
LLLALEPHKEQYKEFQDLNQEVMNLDDILKK- Isotype
- IgG
- Antibody clone number
- 2F6-1A11
- Storage
- Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
Submitted references Fam65b is a new transcriptional target of FOXO1 that regulates RhoA signaling for T lymphocyte migration.
Rougerie P, Largeteau Q, Megrelis L, Carrette F, Lejeune T, Toffali L, Rossi B, Zeghouf M, Cherfils J, Constantin G, Laudanna C, Bismuth G, Mangeney M, Delon J
Journal of immunology (Baltimore, Md. : 1950) 2013 Jan 15;190(2):748-55
Journal of immunology (Baltimore, Md. : 1950) 2013 Jan 15;190(2):748-55
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western Blot analysis of C6orf32 expression in transfected 293T cell line by C6orf32 monoclonal antibody (M02), clone 2F6-1A11.Lane 1: C6orf32 transfected lysate(66 KDa).Lane 2: Non-transfected lysate.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Detection limit for recombinant GST tagged FAM65B is 0.3 ng/ml as a capture antibody.
- Validation comment
- Sandwich ELISA (Recombinant protein)
- Protocol
- Protocol
