Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
Submit
Validation data
Reference
Comment
Report error
- Product number
- TA504564 - Provider product page
- Provider
- OriGene
- Proper citation
- OriGene Cat#TA504564, RRID:AB_2622467
- Product name
- CYB5R1 mouse monoclonal antibody, clone OTI4B7 (formerly 4B7)
- Antibody type
- Monoclonal
- Description
- CYB5R1 mouse monoclonal antibody, clone OTI4B7 (formerly 4B7)
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- CYB5R1
- Isotype
- IgG
- Antibody clone number
- OTI4B7
- Vial size
- 100 µl
- Concentration
- 0.74 mg/ml
No comments: Submit comment
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CYB5R1 (RC205833, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CYB5R1.
- Validation comment
- WB
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot analysis of extracts (10ug) from 1 cell line by using anti-CYB5R1 monoclonal antibody.(1:200)
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Anti-CYB5R1 mouse monoclonal antibody (TA504564) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY CYB5R1(RC205833).
- Validation comment
- IF
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-CYB5R1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504564)
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-CYB5R1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504564)
- Validation comment
- IHC
