HPA030083

antibody from Atlas Antibodies

Targeting: SF3A1 Prp21, PRPF21, SAP114, SF3a120

Western blot (Enhanced validation)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Enhanced validation)

Antibody Data

Product number

HPA030083

Provider

Atlas Antibodies

Proper citation

Atlas Antibodies Cat#HPA030083, RRID:AB_10602183

Product name

Anti-SF3A1

Antibody type

Polyclonal

Reactivity

Human

Host

Rabbit

Conjugate

Unconjugated

Antigen sequence

YPPPEVRNIVDKTASFVARNGPEFEARIRQNEINN
PKFNFLNPNDPYHAYYRHKVSEFKEGKAQEPSAAI
PKVMQQQQQTTQQQLPQKVQAQVIQETIVPKEPPP
EFEFIADPPSISAFDLDVVKLTAQFVARNGRQFLT
QLMQKEQRNYQFDFLRPQHSLFNYFTKLVEQYTKI


Isotype

IgG

Vial size

100 µl

Storage

Store at +4°C for short term storage. Long time storage is recommended at -20°C.

Western blot

Enhanced validation

Submitted by

klas2

Enhanced method

Genetic validation

Main image

Experimental details

Western blot of cell lysate from U-2 OS cells transfected with either siRNA targeting SF3A1 or control siRNA. Lane 1: Marker (250, 130, 95, 72, 55, 36, 28, 17, 10) Lane 2: Cell lysate from U-2OS cells transfected with siRNA targeting SF3A1 Lane 3: N/A Lane 4: Cell lysate from U-2OS cells transfected with control siRNA Right image, lane 1-4: loading control

Sample type

U-2 OS

Primary Ab dilution

1:98

Conjugate

Horseradish Peroxidase

Secondary Ab

Secondary Ab

Secondary Ab dilution

1:3000

Knockdown/Genetic Approaches Application

Western blot

KD Reagent Type

siRNA

Downregulation

>75%

KD Reagent Provider

Thermo Fisher Scientific

KD Reagent Prod no

s20116

KD Reagent Prod page

KD Reagent Prod page

KD Reagent Prod no 2

s20117

KD Reagent Page 2

KD Reagent Page 2

KD Reagent Page 3

KD Reagent Page 3

KD Reagent Prod no 3

s20118

Antibody Lot Number

R30292

Appendix

566d598becbc2.pdf

Show more

Supportive validation

Submitted by

Atlas Antibodies (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Western blot analysis in U2OS cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-SF3A1 antibody. Remaining relative intensity is presented.

Immunocytochemistry

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunofluorescent staining of human cell line U-2 OS shows localization to nuclear speckles.

Sample type

HUMAN

Immunohistochemistry

Enhanced validation

Submitted by

Atlas Antibodies (provider)

Enhanced method

Independent antibody validation

Main image

Experimental details

Immunohistochemical staining of human cerebral cortex, colon, kidney and testis using Anti-SF3A1 antibody HPA030083 (A) shows similar protein distribution across tissues to independent antibody HPA000690 (B).

Antibody #2 product nr

HPA000690

Antibody provider

Atlas Antibodies

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Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human breast shows strong nuclear positivity in glandular cells.

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human kidney using Anti-SF3A1 antibody HPA030083.

Sample type

HUMAN

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human cerebral cortex using Anti-SF3A1 antibody HPA030083.

Sample type

HUMAN

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human colon using Anti-SF3A1 antibody HPA030083.

Sample type

HUMAN

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human testis using Anti-SF3A1 antibody HPA030083.

Sample type

HUMAN