NBP1-87214

antibody from Novus Biologicals

Targeting: SF3A1 Prp21, PRPF21, SAP114, SF3a120

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

NBP1-87214

Provider

Novus Biologicals

Proper citation

Novus Cat#NBP1-87214, RRID:AB_11008226

Product name

Rabbit Polyclonal SF3A1 Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified. Specificity of human, mouse, rat SF3A1 antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

0.1 ml

Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

References

Submitted references

The nuclear splicing factor RNA binding motif 5 promotes caspase activation in human neuronal cells, and increases after traumatic brain injury in mice.

Jackson TC, Du L, Janesko-Feldman K, Vagni VA, Dezfulian C, Poloyac SM, Jackson EK, Clark RS, Kochanek PM
Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism 2015 Mar 31;35(4):655-66

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: SF3A1 Antibody [NBP1-87214] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: SF3A1 Antibody [NBP1-87214] - Analysis in U-251MG cells transfected with control siRNA, target specific siRNA probe #1. Remaining relative intensity is presented. Loading control: Anti-GAPDH.

Immunohistochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: SF3A1 Antibody [NBP1-87214] - Staining of human kidney using Anti-SF3A1 antibody.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: SF3A1 Antibody [NBP1-87214] - Staining of human colon using Anti-SF3A1 antibody.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: SF3A1 Antibody [NBP1-87214] - Staining of human cerebral cortex, colon, kidney and testis using Anti-SF3A1 antibody NBP1-87214 (A) shows similar protein distribution across tissues to independent antibody NBP1-87215 (B).

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: SF3A1 Antibody [NBP1-87214] - Staining of human testis using Anti-SF3A1 antibody.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: SF3A1 Antibody [NBP1-87214] - Staining of human cerebral cortex using Anti-SF3A1 antibody.