Antibody data
- Antibody Data
- Antigen structure
- References [8]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [4]
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Validation data
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- Product number
- NBP1-84449 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-84449, RRID:AB_11028306
- Product name
- Rabbit Polyclonal PLA2R1 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Specificity of human PLA2R1 antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references Prevalence of Enhanced Granular Expression of Thrombospondin Type-1 Domain-Containing 7A in the Glomeruli of Japanese Patients with Idiopathic Membranous Nephropathy.
Membranous Nephropathy Associated With Immunological Disorder-Related Liver Disease: A Retrospective Study of 10 Cases.
Prevalence of anti-phospholipase A2 receptor antibodies in Japanese patients with membranous nephropathy.
Kidney biopsy is a sensitive tool for retrospective diagnosis of PLA2R-related membranous nephropathy.
PLA2R1 kills cancer cells by inducing mitochondrial stress.
Enhanced expression of the M-type phospholipase A2 receptor in glomeruli correlates with serum receptor antibodies in primary membranous nephropathy.
Autoantibodies specific for the phospholipase A2 receptor in recurrent and De Novo membranous nephropathy.
PLA2R autoantibodies and PLA2R glomerular deposits in membranous nephropathy.
Iwakura T, Ohashi N, Kato A, Baba S, Yasuda H
PloS one 2015;10(9):e0138841
PloS one 2015;10(9):e0138841
Membranous Nephropathy Associated With Immunological Disorder-Related Liver Disease: A Retrospective Study of 10 Cases.
Dauvergne M, Moktefi A, Rabant M, Vigneau C, Kofman T, Burtey S, Corpechot C, Stehlé T, Desvaux D, Rioux-Leclercq N, Rouvier P, Knebelmann B, Boffa JJ, Frouget T, Daugas E, Jablonski M, Dahan K, Brocheriou I, Remy P, Grimbert P, Lang P, Chazouilleres O, Sahali D, Audard V
Medicine 2015 Jul;94(30):e1243
Medicine 2015 Jul;94(30):e1243
Prevalence of anti-phospholipase A2 receptor antibodies in Japanese patients with membranous nephropathy.
Akiyama S, Akiyama M, Imai E, Ozaki T, Matsuo S, Maruyama S
Clinical and experimental nephrology 2015 Aug;19(4):653-60
Clinical and experimental nephrology 2015 Aug;19(4):653-60
Kidney biopsy is a sensitive tool for retrospective diagnosis of PLA2R-related membranous nephropathy.
Svobodova B, Honsova E, Ronco P, Tesar V, Debiec H
Nephrology, dialysis, transplantation : official publication of the European Dialysis and Transplant Association - European Renal Association 2013 Jul;28(7):1839-44
Nephrology, dialysis, transplantation : official publication of the European Dialysis and Transplant Association - European Renal Association 2013 Jul;28(7):1839-44
PLA2R1 kills cancer cells by inducing mitochondrial stress.
Augert A, Vindrieux D, Girard CA, Le Calvé B, Gras B, Ferrand M, Bouchet BP, Puisieux A, de Launoit Y, Simonnet H, Lambeau G, Bernard D
Free radical biology & medicine 2013 Dec;65:969-977
Free radical biology & medicine 2013 Dec;65:969-977
Enhanced expression of the M-type phospholipase A2 receptor in glomeruli correlates with serum receptor antibodies in primary membranous nephropathy.
Hoxha E, Kneißler U, Stege G, Zahner G, Thiele I, Panzer U, Harendza S, Helmchen UM, Stahl RA
Kidney international 2012 Oct;82(7):797-804
Kidney international 2012 Oct;82(7):797-804
Autoantibodies specific for the phospholipase A2 receptor in recurrent and De Novo membranous nephropathy.
Debiec H, Martin L, Jouanneau C, Dautin G, Mesnard L, Rondeau E, Mousson C, Ronco P
American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons 2011 Oct;11(10):2144-52
American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons 2011 Oct;11(10):2144-52
PLA2R autoantibodies and PLA2R glomerular deposits in membranous nephropathy.
Debiec H, Ronco P
The New England journal of medicine 2011 Feb 17;364(7):689-90
The New England journal of medicine 2011 Feb 17;364(7):689-90
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: PLA2R1 Antibody [NBP1-84449] - Analysis in human kidney tissue.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: PLA2R1 Antibody [NBP1-84449] - Analysis in control (vector only transfected HEK293T lysate) and PLA2R1 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: PLA2R1 Antibody [NBP1-84449] - Immunohistochemical staining of human pancreas shows no positivity in exocrine glandular cells as expected.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: PLA2R1 Antibody [NBP1-84449] - Staining of human kidney shows weak membranous positivity in cells in glomeruli.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: PLA2R1 Antibody [NBP1-84449] - Staining in human kidney and pancreas tissues . Corresponding PLA2R1 RNA-seq data are presented for the same tissues.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: PLA2R1 Antibody [NBP1-84449] - Staining o human kidney (idiopathic membranous nephropathy) shows strong membranous positivity in cells in glomeruli.