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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- ELISA [1]
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- Product number
- TA347281 - Provider product page
- Provider
- OriGene
- Product name
- Rabbit Polyclonal AML-ETO Antibody
- Antibody type
- Polyclonal
- Description
- Rabbit Polyclonal AML-ETO Antibody
- Host
- Rabbit
- Conjugate
- Unconjugated
- Epitope
- RUNX1T1
- Isotype
- IgG
- Antibody clone number
- NULL
- Vial size
- 50 µg
- Concentration
- 0.2 ug/ul
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- WB was performed on seven GST-fusion proteins containing different fragments of AML-ETO (1-7) with the ab at 1:1,000. The antibody raised against AML-ETO recognizes the ETO part (lane 2) of the fusion protein. C: WB was performed on nuclear extracts from KAS-6/1 cells (human myeloma cell line) and SKNO-1 cells (human acute myeloblastic leukaemia) with the ab against AML-ETO at 1:1,000 . The location of AML-ETO and a presumed splice variant (missing 106 C-terminal amino acids) are indicated.
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Determination of the antibody titer To determine the titer of the antibody, an ELISA was performed using a serial dilution of antibody against AML-ETO, crude serum and flow through in antigen coated wells. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:5,250.
- Validation comment
- ELISA
