Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [2]
- Flow cytometry [3]
Submit
Validation data
Reference
Comment
Report error
- Product number
- TA502271 - Provider product page
- Provider
- OriGene
- Proper citation
- OriGene Cat#TA502271, RRID:AB_11141552
- Product name
- PIP4K2A mouse monoclonal antibody, clone OTI3D3 (formerly 3D3)
- Antibody type
- Monoclonal
- Description
- PIP4K2A mouse monoclonal antibody, clone OTI3D3 (formerly 3D3)
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- PIP4K2A
- Isotype
- IgG
- Antibody clone number
- OTI3D3
- Vial size
- 100 µl
- Concentration
- 0.9 mg/ml
No comments: Submit comment
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PIP4K2A (RC205243, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PIP4K2A.
- Validation comment
- WB
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot analysis of extracts (10ug) from 3 different cell lines by using anti-PIP4K2A monoclonal antibody.(1:200)
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-PIP4K2A mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA502271)
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-PIP4K2A mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA502271)
- Validation comment
- IHC
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Flow cytometric Analysis of Jurkat cells, using anti-PIP4K2A antibody(TA502271),(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).
- Validation comment
- FC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells transfected with either RC205243 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-PIP4K2A antibody(TA502271), and then analyzed by flow cytometry.
- Validation comment
- FC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Flow cytometric Analysis of Hela cells, using anti-PIP4K2A antibody(TA502271),(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).
- Validation comment
- FC
