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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunoprecipitation [1]
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- Product number
- H00002189-M01 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#H00002189-M01, RRID:AB_425425
- Product name
- FANCG monoclonal antibody (M01), clone 2C8
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody raised against a full length recombinant FANCG.
- Antigen sequence
MSRQTTSVGSSCLDLWREKNDRLVRQAKVAQNSGL
TLRRQQLAQDALEGLRGLLHSLQGLPAAVPVLPLE
LTVTCNFIILRASLAQGFTEDQAQDIQRSLERVLE
TQEQQGPRLEQGLRELWDSVLRASCLLPELLSALH
RLVGLQAALWLSADRLGDLALLLETLNGSQSGASK
DLLLLLKTWSPPAEELDAPLTLQDAQGLKDVLLTA
FAYRQGLQELITGNPDKALSSLHEAASGLCPRPVL
VQVYTALGSCHRKMGNPQRALLYLVAALKEGSAWG
PPLLEASRLYQQLGDTTAELESLELLVEALNVPCS
SKAPQFLIEVELLLPPPDLASPLHCGTQSQTKHIL
ASRCLQTGRAGDAAEHYLDLLALLLDSSEPRFSPP
PSPPGPCMPEVFLEAAVALIQAGRAQDALTLCEEL
LSRTSSLLPKMSRLWEDARKGTKELPYCPLWVSAT
HLLQGQAWVQLGAQKVAISEFSRCLELLFRATPEE
KEQGAAFNCEQGCKSDAALQQLRAAALISRGLEWV
ASGQDTKALQDFLLSVQMCPGNRDTYFHLLQTLKR
LDRRDEATALWWRLEAQTKGSHEDALWSLPLYLES
YLSWIRPSDRDAFLEEFRTSLPKSCDL- Isotype
- IgG
- Antibody clone number
- 2C8
- Storage
- Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
Submitted references Areca nut induces miR-23a and inhibits repair of DNA double-strand breaks by targeting FANCG.
Stimulation of homology-directed repair at I-SceI-induced DNA breaks during the permissive life cycle of human cytomegalovirus.
Four human FANCG polymorphic variants show normal biological function in hamster CHO cells.
Tsai YS, Lin CS, Chiang SL, Lee CH, Lee KW, Ko YC
Toxicological sciences : an official journal of the Society of Toxicology 2011 Oct;123(2):480-90
Toxicological sciences : an official journal of the Society of Toxicology 2011 Oct;123(2):480-90
Stimulation of homology-directed repair at I-SceI-induced DNA breaks during the permissive life cycle of human cytomegalovirus.
Kulkarni AS, Fortunato EA
Journal of virology 2011 Jun;85(12):6049-54
Journal of virology 2011 Jun;85(12):6049-54
Four human FANCG polymorphic variants show normal biological function in hamster CHO cells.
Hinz JM, Nham PB, Yamada NA, Tebbs RS, Salazar EP, Hinz AK, Mohrenweiser HW, Jones IM, Thompson LH
Mutation research 2006 Dec 1;602(1-2):34-42
Mutation research 2006 Dec 1;602(1-2):34-42
No comments: Submit comment
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- FANCG monoclonal antibody (M01), clone 2C8 Western Blot analysis of FANCG expression in Hela S3 NE ( Cat # L013V3 ).
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western Blot analysis of FANCG expression in transfected 293T cell line by FANCG monoclonal antibody (M01), clone 2C8.Lane 1: FANCG transfected lysate(68.6 KDa).Lane 2: Non-transfected lysate.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunofluorescence of monoclonal antibody to FANCG on HeLa cell. [antibody concentration 10 ug/ml]
- Validation comment
- Immunofluorescence
- Protocol
- Protocol
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunoprecipitation of FANCG transfected lysate using anti-FANCG monoclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with FANCG MaxPab rabbit polyclonal antibody.
- Validation comment
- Immunoprecipitation
- Protocol
- Protocol
